1Q36

EPSP synthase (Asp313Ala) liganded with tetrahedral reaction intermediate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0003866	molecular_function	3-phosphoshikimate 1-carboxyvinyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008652	biological_process	amino acid biosynthetic process
A	0009073	biological_process	aromatic amino acid family biosynthetic process
A	0009423	biological_process	chorismate biosynthetic process
A	0016740	molecular_function	transferase activity
A	0016765	molecular_function	transferase activity, transferring alkyl or aryl (other than methyl) groups

Functional Information from PDB Data

site_id	AC1
Number of Residues	24
Details	BINDING SITE FOR RESIDUE SKP A 600

Chain	Residue
A	LYS22
A	GLN171
A	SER197
A	TYR200
A	ASN336
A	LYS340
A	GLU341
A	ARG344
A	HIS385
A	ARG386
A	LYS411
A	SER23
A	HOH621
A	HOH629
A	HOH641
A	HOH681
A	HOH712
A	ARG27
A	ASN94
A	GLY96
A	THR97
A	ARG124
A	SER169
A	SER170

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE FMT A 601

Chain	Residue
A	LYS373
A	LEU374
A	SER397
A	ASP398
A	HOH740
A	HOH907
A	HOH915

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 602

Chain	Residue
A	THR58
A	VAL62
A	SER63
A	TYR64
A	HOH904
A	HOH1058

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 603

Chain	Residue
A	THR5
A	LEU143
A	ARG152
A	PHE376
A	THR402

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 604

Chain	Residue
A	THR65
A	LEU66
A	SER67
A	ARG72
A	HOH980
A	HOH1046

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 605

Chain	Residue
A	LEU60
A	HIS83
A	HOH781
A	HOH813
A	HOH986
A	HOH1147

---

site_id	AC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE FMT A 606

Chain	Residue
A	GLY264
A	ILE265
A	GLY266
A	SER269
A	HOH747
A	HOH888
A	HOH1022
A	HOH1028

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 607

Chain	Residue
A	LEU88
A	HOH709
A	HOH787
A	HOH813
A	HOH889

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 608

Chain	Residue
A	LEU135
A	GLY137
A	HOH824
A	HOH847
A	HOH1138

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 609

Chain	Residue
A	ALA380
A	TYR382
A	HOH701
A	HOH792
A	HOH994

---

Functional Information from PROSITE/UniProt

site_id	PS00104
Number of Residues	15
Details	EPSP_SYNTHASE_1 EPSP synthase signature 1. LFlGNAGTAMRpLaA

Chain	Residue	Details
A	LEU90-ALA104

---

site_id	PS00885
Number of Residues	19
Details	EPSP_SYNTHASE_2 EPSP synthase signature 2. RvKETDRLfAMateLrkVG

Chain	Residue	Details
A	ARG338-GLY356

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000305|PubMed:13129913

Chain	Residue	Details
A	ALA313

---

site_id	SWS_FT_FI2
Number of Residues	7
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00210

Chain	Residue	Details
A	LYS22
A	GLY96
A	ARG124
A	GLN171
A	ARG344
A	ARG386
A	LYS411

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:11171958, ECO:0007744|PDB:1G6S

Chain	Residue	Details
A	SER23
A	ARG27
A	SER169
A	SER170
A	SER197
A	ALA313
A	ASN336
A	LYS340

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Modified by bromopyruvate => ECO:0000269|PubMed:11171958

Chain	Residue	Details
A	CYS408
A	LYS411

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1g6t

Chain	Residue	Details
A	LYS22
A	ALA313
A	LYS411
A	HIS385
A	GLU341

---

site_id	MCSA1
Number of Residues	7
Details	M-CSA 457

Chain	Residue	Details
A	ASP49	metal ligand
A	ASN94	metal ligand
A	ALA313	electrostatic stabiliser, proton shuttle (general acid/base)
A	GLU341	electrostatic stabiliser, metal ligand, proton shuttle (general acid/base)
A	HIS385	steric role
A	ARG386	transition state stabiliser
A	LYS411	steric role

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