1Q36
EPSP synthase (Asp313Ala) liganded with tetrahedral reaction intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.810, 85.049, 87.545 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.155 |
Rwork | 0.155 |
R-free | 0.18700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g6t |
RMSD bond length | 0.016 |
RMSD bond angle | 1.794 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.059 | 0.330 |
Number of reflections | 55905 | |
<I/σ(I)> | 17.4 | 5.3 |
Completeness [%] | 97.0 | 95.1 |
Redundancy | 5.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | sodium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |