1PWW

Crystal structure of Anthrax Lethal Factor active site mutant protein complexed with an optimised peptide substrate in the presence of zinc.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004222	molecular_function	metalloendopeptidase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0008270	molecular_function	zinc ion binding
A	0016787	molecular_function	hydrolase activity
A	0030430	cellular_component	host cell cytoplasm
A	0035821	biological_process	modulation of process of another organism
A	0044164	cellular_component	host cell cytosol
A	0046872	molecular_function	metal ion binding
A	0090729	molecular_function	toxin activity
B	0003824	molecular_function	catalytic activity
B	0004222	molecular_function	metalloendopeptidase activity
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0006508	biological_process	proteolysis
B	0008233	molecular_function	peptidase activity
B	0008237	molecular_function	metallopeptidase activity
B	0008270	molecular_function	zinc ion binding
B	0016787	molecular_function	hydrolase activity
B	0030430	cellular_component	host cell cytoplasm
B	0035821	biological_process	modulation of process of another organism
B	0044164	cellular_component	host cell cytosol
B	0046872	molecular_function	metal ion binding
B	0090729	molecular_function	toxin activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN A 9001

Chain	Residue
A	HIS686
A	HIS690
A	TYR728
A	GLU735
C	PRO10

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN B 9002

Chain	Residue
D	TYR9
D	PRO10
B	HIS686
B	HIS690
B	TYR728
B	GLU735

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	424
Details	Domain: {"description":"ATLF-like 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	36
Details	Repeat: {"description":"1","evidences":[{"source":"PubMed","id":"11700563","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	30
Details	Repeat: {"description":"2","evidences":[{"source":"PubMed","id":"11700563","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	36
Details	Repeat: {"description":"3","evidences":[{"source":"PubMed","id":"11700563","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	390
Details	Domain: {"description":"ATLF-like 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	68
Details	Region: {"description":"IIA","evidences":[{"source":"PubMed","id":"11700563","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	326
Details	Region: {"description":"IIB","evidences":[{"source":"PubMed","id":"11700563","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	448
Details	Region: {"description":"IV","evidences":[{"source":"PubMed","id":"11700563","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU01339","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"19651869","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9573135","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01339","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11700563","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14718924","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14718925","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15911756","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22342144","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25372673","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26492514","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26578066","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1J7N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PWP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PWQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PWU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PWW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1YQY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DV8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PKR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PKS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PKT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PKV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PKW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4WF6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4XM6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4XM7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5D1S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5D1T","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	17
Details	Repeat: {"description":"5","evidences":[{"source":"PubMed","id":"11700563","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1pwv

Chain	Residue	Details
A	CYS687
A	TYR728

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1pwv

Chain	Residue	Details
B	CYS687
B	TYR728

---

site_id	MCSA1
Number of Residues	5
Details	M-CSA 641

Chain	Residue	Details
A	HIS686	metal ligand
A	CYS687	proton acceptor, proton donor
A	HIS690	metal ligand
A	TYR728	electrostatic stabiliser
A	GLU735	metal ligand

---

site_id	MCSA2
Number of Residues	5
Details	M-CSA 641

Chain	Residue	Details
B	HIS686	metal ligand
B	CYS687	proton acceptor, proton donor
B	HIS690	metal ligand
B	TYR728	electrostatic stabiliser
B	GLU735	metal ligand

---