1PT2
Crystal structure of levansucrase (E342A) complexed with sucrose
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005576 | cellular_component | extracellular region |
| A | 0009758 | biological_process | carbohydrate utilization |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050053 | molecular_function | levansucrase activity |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile => ECO:0000305|PubMed:14517548 |
| Chain | Residue | Details |
| A | ASP86 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:14517548 |
| Chain | Residue | Details |
| A | ALA342 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 7 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:14517548, ECO:0007744|PDB:1PT2, ECO:0007744|PDB:3BYN |
| Chain | Residue | Details |
| A | TRP85 | |
| A | ASP86 | |
| A | SER164 | |
| A | ARG246 | |
| A | ASP247 | |
| A | GLU340 | |
| A | ARG360 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:14517548, ECO:0000269|PubMed:18596022, ECO:0000269|PubMed:32553967, ECO:0000269|PubMed:33303628, ECO:0007744|PDB:1OYG, ECO:0007744|PDB:1PT2, ECO:0007744|PDB:2VDT, ECO:0007744|PDB:3BYJ, ECO:0007744|PDB:3BYK, ECO:0007744|PDB:3BYN, ECO:0007744|PDB:6PWQ, ECO:0007744|PDB:6VHQ |
| Chain | Residue | Details |
| A | ASP241 | |
| A | GLN272 | |
| A | LEU308 | |
| A | ASN310 | |
| A | ASP339 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | SITE: Transition state stabilizer => ECO:0000305|PubMed:14517548 |
| Chain | Residue | Details |
| A | ASP247 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 875 |
| Chain | Residue | Details |
| A | ASP86 | covalent catalysis |
| A | SER164 | steric locator |
| A | ASP247 | electrostatic stabiliser |
| A | ALA342 | proton shuttle (general acid/base) |
