1PT2

Crystal structure of levansucrase (E342A) complexed with sucrose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-04-01
Detector	MAC Science DIP-2030B
Spacegroup name	P 21 21 21
Unit cell lengths	51.241, 66.951, 123.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.100
R-factor	0.1909
Rwork	0.189
R-free	0.23200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oyg
RMSD bond length	0.007
RMSD bond angle	1.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.070	2.070
Rmerge	0.065 *	0.183 *
Total number of observations	202902 *
Number of reflections	26171
Completeness [%]	98.2	96.9
Redundancy	4.0 *	3.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	60 (mg/ml)
2	1	2	diammonium phosphate	1 (mM)
3	1	2	sodium acetate	1 (mM)	pH6.3