1PT2
Crystal structure of levansucrase (E342A) complexed with sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-04-01 |
Detector | MAC Science DIP-2030B |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.241, 66.951, 123.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.100 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.23200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oyg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.140 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.065 * | 0.183 * |
Total number of observations | 202902 * | |
Number of reflections | 26171 | |
Completeness [%] | 98.2 | 96.9 |
Redundancy | 4.0 * | 3.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 6.3 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 60 (mg/ml) | |
2 | 1 | 2 | diammonium phosphate | 1 (mM) | |
3 | 1 | 2 | sodium acetate | 1 (mM) | pH6.3 |