1PNT

CRYSTAL STRUCTURE OF BOVINE HEART PHOSPHOTYROSYL PHOSPHATASE AT 2.2 ANGSTROMS RESOLUTION

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003993	molecular_function	acid phosphatase activity
A	0004721	molecular_function	phosphoprotein phosphatase activity
A	0004725	molecular_function	protein tyrosine phosphatase activity
A	0004726	molecular_function	non-membrane spanning protein tyrosine phosphatase activity
A	0005737	cellular_component	cytoplasm
A	0006470	biological_process	protein dephosphorylation
A	0016787	molecular_function	hydrolase activity
A	0016791	molecular_function	phosphatase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PO4 A 158

Chain	Residue
A	LEU13
A	GLY14
A	ASN15
A	ILE16
A	CYS17
A	ARG18
A	ASP129
A	CYS12

---

site_id	ACT
Number of Residues	8
Details	ACTIVE SITE

Chain	Residue
A	CYS12
A	LEU13
A	GLY14
A	ASN15
A	ILE16
A	CYS17
A	ARG18
A	SER19

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:8052313, ECO:0000269|PubMed:8132604, ECO:0000269|PubMed:8319676

Chain	Residue	Details
A	LEU13

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:8052313, ECO:0000269|PubMed:8319676

Chain	Residue	Details
A	SER19

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000269|PubMed:8052313

Chain	Residue	Details
A	PRO130

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:2644264

Chain	Residue	Details
A	GLU2

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P24666

Chain	Residue	Details
A	TYR132
A	GLY133

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 462

Chain	Residue	Details
A	LEU13	covalent catalysis
A	ILE16	activator, electrostatic stabiliser
A	ARG18	steric role
A	SER19	transition state stabiliser
A	PRO20	activator, electrostatic stabiliser
A	PRO130	proton shuttle (general acid/base)

---