1PNT

CRYSTAL STRUCTURE OF BOVINE HEART PHOSPHOTYROSYL PHOSPHATASE AT 2.2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	95.300, 43.300, 41.200
Unit cell angles	90.00, 113.50, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.166
Rwork	0.166
RMSD bond length	0.010
RMSD bond angle	2.090
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.060 *
Completeness [%]	90.0 *
Redundancy	3.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		Zhang, M., (1994) J.Mol.Biol., 238, 281. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20 (%)
2	1	reservoir	Tris-HCl	50 (mM)
3	1	reservoir		50 (mM)