1P6W
Crystal structure of barley alpha-amylase isozyme 1 (AMY1) in complex with the substrate analogue, methyl 4I,4II,4III-tri-thiomaltotetraoside (thio-DP4)
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004556 | molecular_function | alpha-amylase activity |
A | 0005509 | molecular_function | calcium ion binding |
A | 0005576 | cellular_component | extracellular region |
A | 0005975 | biological_process | carbohydrate metabolic process |
A | 0005983 | biological_process | starch catabolic process |
A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
A | 0043169 | molecular_function | cation binding |
A | 0046872 | molecular_function | metal ion binding |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1 |
Details | ACT_SITE: Nucleophile => ECO:0000305|PubMed:16030022, ECO:0000305|PubMed:7901200 |
Chain | Residue | Details |
A | ASP180 |
site_id | SWS_FT_FI2 |
Number of Residues | 1 |
Details | ACT_SITE: Proton donor => ECO:0000305|PubMed:7901200 |
Chain | Residue | Details |
A | GLU205 |
site_id | SWS_FT_FI3 |
Number of Residues | 2 |
Details | BINDING: BINDING => ECO:0000305|PubMed:12906828 |
Chain | Residue | Details |
A | HIS45 | |
A | ARG178 |
site_id | SWS_FT_FI4 |
Number of Residues | 7 |
Details | BINDING: BINDING => ECO:0000305|PubMed:16030022 |
Chain | Residue | Details |
A | TYR52 | |
A | TRP207 | |
A | ASN209 | |
A | LYS271 | |
A | HIS290 | |
A | GLN296 | |
A | TRP402 |
site_id | SWS_FT_FI5 |
Number of Residues | 13 |
Details | BINDING: BINDING => ECO:0000269|PubMed:12906828, ECO:0000269|PubMed:16030022, ECO:0000269|PubMed:17803687, ECO:0000269|PubMed:18588886 |
Chain | Residue | Details |
A | ASN92 | |
A | PHE144 | |
A | ALA147 | |
A | ASP149 | |
A | GLY184 | |
A | GLU109 | |
A | THR112 | |
A | ASP114 | |
A | ASP118 | |
A | ASP128 | |
A | ASP139 | |
A | ALA142 | |
A | ASP143 |
site_id | SWS_FT_FI6 |
Number of Residues | 4 |
Details | BINDING: BINDING => ECO:0000305|PubMed:12906828, ECO:0000305|PubMed:16030022 |
Chain | Residue | Details |
A | GLN227 | |
A | GLY277 | |
A | LYS375 | |
A | THR392 |
site_id | SWS_FT_FI7 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000305|PubMed:16030022, ECO:0000305|PubMed:17803687 |
Chain | Residue | Details |
A | ASP234 |
site_id | SWS_FT_FI8 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000305|PubMed:12906828, ECO:0000305|PubMed:16030022, ECO:0000305|PubMed:17803687, ECO:0000305|PubMed:18588886 |
Chain | Residue | Details |
A | TYR380 |
site_id | SWS_FT_FI9 |
Number of Residues | 1 |
Details | SITE: Transition state stabilizer => ECO:0000305|PubMed:7901200 |
Chain | Residue | Details |
A | ASP291 |
Catalytic Information from CSA
site_id | CSA1 |
Number of Residues | 2 |
Details | Annotated By Reference To The Literature 1amy |
Chain | Residue | Details |
A | ASP180 | |
A | GLU205 |
site_id | CSA2 |
Number of Residues | 2 |
Details | Annotated By Reference To The Literature 1amy |
Chain | Residue | Details |
A | ASP234 | |
A | ASP180 |
site_id | CSA3 |
Number of Residues | 4 |
Details | Annotated By Reference To The Literature 1amy |
Chain | Residue | Details |
A | TRP207 | |
A | ASP291 | |
A | ASP180 | |
A | GLU205 |
site_id | CSA4 |
Number of Residues | 5 |
Details | Annotated By Reference To The Literature 1amy |
Chain | Residue | Details |
A | ASP291 | |
A | ASP180 | |
A | HIS290 | |
A | ARG178 | |
A | GLU205 |
site_id | CSA5 |
Number of Residues | 4 |
Details | Annotated By Reference To The Literature 1amy |
Chain | Residue | Details |
A | ASP291 | |
A | ASP180 | |
A | HIS93 | |
A | GLU205 |
site_id | CSA6 |
Number of Residues | 3 |
Details | Annotated By Reference To The Literature 1amy |
Chain | Residue | Details |
A | ASP291 | |
A | ASP180 | |
A | GLU205 |