1NL7
Z. ramigera biosynthetic thiolase, acetylated enzyme complexed with CoA at pH 9.5
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| A | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| B | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| B | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| C | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0016746 | molecular_function | acyltransferase activity |
| C | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| C | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| D | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0016746 | molecular_function | acyltransferase activity |
| D | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| D | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 9720 |
| Chain | Residue |
| A | SER260 |
| A | ALA262 |
| A | ARG266 |
| A | HOH9957 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 9722 |
| Chain | Residue |
| A | HOH9849 |
| A | LYS298 |
| A | ARG302 |
| A | HOH470 |
| A | HOH537 |
| A | HOH538 |
| site_id | AC3 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE COA A 1393 |
| Chain | Residue |
| A | SCY89 |
| A | LEU148 |
| A | HIS156 |
| A | MET157 |
| A | SER227 |
| A | ALA243 |
| A | GLY244 |
| A | SER247 |
| A | MET288 |
| A | PHE319 |
| A | HIS348 |
| A | HOH409 |
| A | HOH481 |
| A | HOH498 |
| A | HOH513 |
| A | HOH532 |
| A | HOH554 |
| A | HOH9804 |
| A | HOH9858 |
| C | LYS208 |
| D | MET134 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 9719 |
| Chain | Residue |
| B | LYS298 |
| B | ARG302 |
| B | HOH540 |
| B | HOH9928 |
| B | HOH9931 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 9721 |
| Chain | Residue |
| B | SER260 |
| B | ARG266 |
| B | HOH419 |
| B | HOH9883 |
| B | HOH9952 |
| site_id | AC6 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE COA B 5939 |
| Chain | Residue |
| B | SCY89 |
| B | LEU148 |
| B | HIS156 |
| B | MET157 |
| B | ARG220 |
| B | SER227 |
| B | MET228 |
| B | LEU231 |
| B | GLY244 |
| B | SER247 |
| B | MET288 |
| B | ALA318 |
| B | PHE319 |
| B | HIS348 |
| B | HOH467 |
| B | HOH534 |
| B | HOH9824 |
| B | HOH9834 |
| B | HOH9900 |
| B | HOH9919 |
Functional Information from PROSITE/UniProt
| site_id | PS00098 |
| Number of Residues | 19 |
| Details | THIOLASE_1 Thiolases acyl-enzyme intermediate signature. MNQlCGSGLrAValgmqqI |
| Chain | Residue | Details |
| A | MET85-ILE103 |
| site_id | PS00099 |
| Number of Residues | 14 |
| Details | THIOLASE_3 Thiolases active site. GLATLCIGgGmGvA |
| Chain | Residue | Details |
| A | GLY373-ALA386 |
| site_id | PS00737 |
| Number of Residues | 17 |
| Details | THIOLASE_2 Thiolases signature 2. NvnGGaIAiGHPiGaSG |
| Chain | Residue | Details |
| A | ASN338-GLY354 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Acyl-thioester intermediate |
| Chain | Residue | Details |
| A | SCY89 | |
| B | SCY89 | |
| C | SCY89 | |
| D | SCY89 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| A | HIS348 | |
| A | CYS378 | |
| B | HIS348 | |
| B | CYS378 | |
| C | HIS348 | |
| C | CYS378 | |
| D | HIS348 | |
| D | CYS378 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| A | HIS348 | |
| A | GLY380 | |
| A | CYS378 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| B | HIS348 | |
| B | GLY380 | |
| B | CYS378 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| C | HIS348 | |
| C | GLY380 | |
| C | CYS378 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| D | HIS348 | |
| D | GLY380 | |
| D | CYS378 |
| site_id | CSA5 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| A | HIS348 | |
| A | CYS378 |
| site_id | CSA6 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| B | HIS348 | |
| B | CYS378 |
| site_id | CSA7 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| C | HIS348 | |
| C | CYS378 |
| site_id | CSA8 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| D | HIS348 | |
| D | CYS378 |
