1NL7
Z. ramigera biosynthetic thiolase, acetylated enzyme complexed with CoA at pH 9.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-14 |
Detector | MARRESEARCH |
Wavelength(s) | 1.1920 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.374, 79.059, 148.881 |
Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.903 |
R-factor | 0.18147 |
Rwork | 0.179 |
R-free | 0.22269 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m3k |
RMSD bond length | 0.019 |
RMSD bond angle | 1.681 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.000 |
High resolution limit [Å] | 1.903 | 1.903 |
Number of reflections | 149016 | |
<I/σ(I)> | 8.1 | 2.4 |
Completeness [%] | 96.6 | 84.6 |
Redundancy | 2.895 | 2.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | lithium sulphate, ammonium sulphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |