1NHX
PEPCK COMPLEX WITH A GTP-COMPETITIVE INHIBITOR
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0004611 | molecular_function | phosphoenolpyruvate carboxykinase activity |
| A | 0004613 | molecular_function | phosphoenolpyruvate carboxykinase (GTP) activity |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005829 | cellular_component | cytosol |
| A | 0006006 | biological_process | glucose metabolic process |
| A | 0006094 | biological_process | gluconeogenesis |
| A | 0006107 | biological_process | oxaloacetate metabolic process |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0009617 | biological_process | response to bacterium |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016831 | molecular_function | carboxy-lyase activity |
| A | 0017076 | molecular_function | purine nucleotide binding |
| A | 0018105 | biological_process | peptidyl-serine phosphorylation |
| A | 0019543 | biological_process | propionate catabolic process |
| A | 0030145 | molecular_function | manganese ion binding |
| A | 0031406 | molecular_function | carboxylic acid binding |
| A | 0032868 | biological_process | response to insulin |
| A | 0032869 | biological_process | cellular response to insulin stimulus |
| A | 0042593 | biological_process | glucose homeostasis |
| A | 0042594 | biological_process | response to starvation |
| A | 0043382 | biological_process | positive regulation of memory T cell differentiation |
| A | 0043648 | biological_process | dicarboxylic acid metabolic process |
| A | 0045944 | biological_process | positive regulation of transcription by RNA polymerase II |
| A | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| A | 0046327 | biological_process | glycerol biosynthetic process from pyruvate |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0046889 | biological_process | positive regulation of lipid biosynthetic process |
| A | 0046890 | biological_process | regulation of lipid biosynthetic process |
| A | 0051365 | biological_process | cellular response to potassium ion starvation |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0070365 | biological_process | hepatocyte differentiation |
| A | 0071333 | biological_process | cellular response to glucose stimulus |
| A | 0071549 | biological_process | cellular response to dexamethasone stimulus |
| A | 0072350 | biological_process | tricarboxylic acid metabolic process |
| A | 0106264 | molecular_function | protein serine kinase activity (using GTP as donor) |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MN A 701 |
| Chain | Residue |
| A | LYS244 |
| A | HIS264 |
| A | ASP311 |
| A | HOH745 |
| A | HOH746 |
| A | HOH748 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA A 702 |
| Chain | Residue |
| A | HOH846 |
| A | ASN403 |
| A | PEP703 |
| A | HOH845 |
| site_id | AC3 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE PEP A 703 |
| Chain | Residue |
| A | ALA86 |
| A | ARG87 |
| A | TYR235 |
| A | GLY236 |
| A | GLY237 |
| A | LYS244 |
| A | ASN403 |
| A | ARG405 |
| A | PHE485 |
| A | NA702 |
| A | HOH745 |
| A | HOH748 |
| A | HOH847 |
| A | HOH909 |
| A | HOH980 |
| site_id | AC4 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE FTB A 704 |
| Chain | Residue |
| A | ALA287 |
| A | GLY289 |
| A | ASN292 |
| A | LEU293 |
| A | THR343 |
| A | TRP516 |
| A | PHE517 |
| A | PHE525 |
| A | PRO528 |
| A | PHE530 |
| A | ASN533 |
| A | HOH865 |
| A | HOH987 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO A 705 |
| Chain | Residue |
| A | SER449 |
| A | TRP450 |
| A | PRO509 |
| A | LYS510 |
| A | ILE511 |
| A | HOH938 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO A 706 |
| Chain | Residue |
| A | TYR72 |
| A | ILE351 |
| A | GLN352 |
| A | VAL368 |
| A | GLU375 |
| A | HOH708 |
Functional Information from PROSITE/UniProt
| site_id | PS00505 |
| Number of Residues | 9 |
| Details | PEPCK_GTP Phosphoenolpyruvate carboxykinase (GTP) signature. FPSACGKTN |
| Chain | Residue | Details |
| A | PHE284-ASN292 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:11851336, ECO:0000305|PubMed:32322062 |
| Chain | Residue | Details |
| A | CYS288 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 7 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11851336, ECO:0000269|PubMed:14552798, ECO:0000269|PubMed:17532214 |
| Chain | Residue | Details |
| A | ARG87 | |
| A | TYR235 | |
| A | LYS244 | |
| A | HIS264 | |
| A | ASP311 | |
| A | ASN403 | |
| A | PHE530 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P07379 |
| Chain | Residue | Details |
| A | SER286 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11851336 |
| Chain | Residue | Details |
| A | ALA287 | |
| A | ARG405 | |
| A | ARG436 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER19 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | MOD_RES: N6-acetyllysine; by p300/EP300 => ECO:0000269|PubMed:20167786, ECO:0000269|PubMed:21726808 |
| Chain | Residue | Details |
| A | LYS70 | |
| A | LYS71 | |
| A | LYS594 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PKB/AKT1 => ECO:0000269|PubMed:32322062 |
| Chain | Residue | Details |
| A | SER90 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine; by p300/EP300 => ECO:0000269|PubMed:30193097 |
| Chain | Residue | Details |
| A | LYS91 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9Z2V4 |
| Chain | Residue | Details |
| A | SER118 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q16822 |
| Chain | Residue | Details |
| A | THR178 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q16822 |
| Chain | Residue | Details |
| A | SER286 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 3 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P07379 |
| Chain | Residue | Details |
| A | LYS473 | |
| A | LYS521 | |
| A | LYS524 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | a catalytic site defined by CSA, PubMed 9139042, 11724534, 11851336, 14552798 |
| Chain | Residue | Details |
| A | HIS264 | |
| A | ARG405 |
| site_id | MCSA1 |
| Number of Residues | 8 |
| Details | M-CSA 863 |
| Chain | Residue | Details |
| A | ARG87 | electrostatic stabiliser, enhance reactivity |
| A | TYR235 | electrostatic stabiliser, steric role |
| A | LYS244 | metal ligand |
| A | HIS264 | metal ligand |
| A | SER286 | electrostatic stabiliser |
| A | CYS288 | metal ligand |
| A | ASP311 | metal ligand |
| A | ARG405 | electrostatic stabiliser, enhance reactivity |
