1NH3

Human Topoisomerase I Ara-C Complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003917	molecular_function	DNA topoisomerase type I (single strand cut, ATP-independent) activity
A	0005694	cellular_component	chromosome
A	0006265	biological_process	DNA topological change

Functional Information from PROSITE/UniProt

site_id	PS00176
Number of Residues	19
Details	TOPO_IB_1 Topoisomerase (Topo) IB-type active site signature. EnkqialGTSKlnYLdprI

Chain	Residue	Details
A	GLU710-ILE728

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Region: {"description":"Interaction with DNA"}

Chain

Residue

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site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"O-(3'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU01382","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10130","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10841763","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12426403","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12533542","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15165849","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15801827","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16033260","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9488644","evidenceCode":"ECO:0000269"}]}

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site_id	SWS_FT_FI3
Number of Residues	7
Details	Site: {"description":"Interaction with DNA"}

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site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

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site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; by CK2","evidences":[{"source":"PubMed","id":"23185622","evidenceCode":"ECO:0000269"}]}

Chain

Residue

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site_id	SWS_FT_FI6
Number of Residues	3
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI7
Number of Residues	1
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25755297","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1k4t

Chain	Residue	Details
A	ARG488
A	ARG590

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