1NAL
THE THREE-DIMENSIONAL STRUCTURE OF N-ACETYLNEURAMINATE LYASE FROM ESCHERICHIA COLI
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| 1 | 0005737 | cellular_component | cytoplasm |
| 1 | 0005829 | cellular_component | cytosol |
| 1 | 0005975 | biological_process | carbohydrate metabolic process |
| 1 | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| 1 | 0016829 | molecular_function | lyase activity |
| 1 | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| 1 | 0042802 | molecular_function | identical protein binding |
| 1 | 0044010 | biological_process | single-species biofilm formation |
| 2 | 0005737 | cellular_component | cytoplasm |
| 2 | 0005829 | cellular_component | cytosol |
| 2 | 0005975 | biological_process | carbohydrate metabolic process |
| 2 | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| 2 | 0016829 | molecular_function | lyase activity |
| 2 | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| 2 | 0042802 | molecular_function | identical protein binding |
| 2 | 0044010 | biological_process | single-species biofilm formation |
| 3 | 0005737 | cellular_component | cytoplasm |
| 3 | 0005829 | cellular_component | cytosol |
| 3 | 0005975 | biological_process | carbohydrate metabolic process |
| 3 | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| 3 | 0016829 | molecular_function | lyase activity |
| 3 | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| 3 | 0042802 | molecular_function | identical protein binding |
| 3 | 0044010 | biological_process | single-species biofilm formation |
| 4 | 0005737 | cellular_component | cytoplasm |
| 4 | 0005829 | cellular_component | cytosol |
| 4 | 0005975 | biological_process | carbohydrate metabolic process |
| 4 | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| 4 | 0016829 | molecular_function | lyase activity |
| 4 | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| 4 | 0042802 | molecular_function | identical protein binding |
| 4 | 0044010 | biological_process | single-species biofilm formation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 1 302 |
| Chain | Residue |
| 1 | ALA11 |
| 1 | TYR43 |
| 1 | GLY46 |
| 1 | SER47 |
| 1 | THR48 |
| 1 | TYR137 |
| 1 | LYS165 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 2 304 |
| Chain | Residue |
| 2 | GLY46 |
| 2 | SER47 |
| 2 | THR48 |
| 2 | TYR137 |
| 2 | LYS165 |
| 2 | ALA11 |
| 2 | TYR43 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 3 306 |
| Chain | Residue |
| 3 | ALA11 |
| 3 | TYR43 |
| 3 | GLY46 |
| 3 | SER47 |
| 3 | THR48 |
| 3 | TYR137 |
| 3 | LYS165 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 4 308 |
| Chain | Residue |
| 4 | ALA11 |
| 4 | TYR43 |
| 4 | GLY46 |
| 4 | SER47 |
| 4 | THR48 |
| 4 | TYR137 |
| 4 | LYS165 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000305|PubMed:24521460 |
| Chain | Residue | Details |
| 1 | ASN138 | |
| 2 | ASN138 | |
| 3 | ASN138 | |
| 4 | ASN138 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Schiff-base intermediate with substrate => ECO:0000269|PubMed:12711733, ECO:0000269|PubMed:19923724, ECO:0000269|PubMed:24521460, ECO:0000269|PubMed:9047371, ECO:0000305|PubMed:8081752 |
| Chain | Residue | Details |
| 1 | GLN166 | |
| 2 | GLN166 | |
| 3 | GLN166 | |
| 4 | GLN166 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9047371, ECO:0000305|PubMed:12711733, ECO:0007744|PDB:1FDY, ECO:0007744|PDB:1FDZ, ECO:0007744|PDB:1HL2 |
| Chain | Residue | Details |
| 1 | THR48 | |
| 1 | GLY49 | |
| 2 | THR48 | |
| 2 | GLY49 | |
| 3 | THR48 | |
| 3 | GLY49 | |
| 4 | THR48 | |
| 4 | GLY49 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 20 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24521460, ECO:0007744|PDB:4BWL |
| Chain | Residue | Details |
| 1 | SER168 | |
| 2 | THR209 | |
| 3 | SER168 | |
| 3 | TYR190 | |
| 3 | GLU192 | |
| 3 | ILE193 | |
| 3 | THR209 | |
| 4 | SER168 | |
| 4 | TYR190 | |
| 4 | GLU192 | |
| 4 | ILE193 | |
| 1 | TYR190 | |
| 4 | THR209 | |
| 1 | GLU192 | |
| 1 | ILE193 | |
| 1 | THR209 | |
| 2 | SER168 | |
| 2 | TYR190 | |
| 2 | GLU192 | |
| 2 | ILE193 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | SITE: Required to correctly position the proton donor => ECO:0000305|PubMed:24521460 |
| Chain | Residue | Details |
| 1 | THR48 | |
| 1 | TYR111 | |
| 2 | THR48 | |
| 2 | TYR111 | |
| 3 | THR48 | |
| 3 | TYR111 | |
| 4 | THR48 | |
| 4 | TYR111 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dhp |
| Chain | Residue | Details |
| 1 | LEU142 | |
| 1 | TYR137 | |
| 1 | LYS165 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dhp |
| Chain | Residue | Details |
| 2 | LEU142 | |
| 2 | TYR137 | |
| 2 | LYS165 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dhp |
| Chain | Residue | Details |
| 3 | LEU142 | |
| 3 | TYR137 | |
| 3 | LYS165 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dhp |
| Chain | Residue | Details |
| 4 | LEU142 | |
| 4 | TYR137 | |
| 4 | LYS165 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dhp |
| Chain | Residue | Details |
| 1 | THR48 | |
| 1 | SER47 | |
| 1 | LYS165 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dhp |
| Chain | Residue | Details |
| 2 | THR48 | |
| 2 | SER47 | |
| 2 | LYS165 |
| site_id | CSA7 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dhp |
| Chain | Residue | Details |
| 3 | THR48 | |
| 3 | SER47 | |
| 3 | LYS165 |
| site_id | CSA8 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dhp |
| Chain | Residue | Details |
| 4 | THR48 | |
| 4 | SER47 | |
| 4 | LYS165 |
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 553 |
| Chain | Residue | Details |
| 1 | ASN138 | proton acceptor, proton donor, proton relay |
| 1 | GLN166 | covalently attached, electron pair acceptor, electron pair donor, nucleofuge, nucleophile, proton acceptor |
| site_id | MCSA2 |
| Number of Residues | 2 |
| Details | M-CSA 553 |
| Chain | Residue | Details |
| 2 | ASN138 | proton acceptor, proton donor, proton relay |
| 2 | GLN166 | covalently attached, electron pair acceptor, electron pair donor, nucleofuge, nucleophile, proton acceptor |
| site_id | MCSA3 |
| Number of Residues | 2 |
| Details | M-CSA 553 |
| Chain | Residue | Details |
| 3 | ASN138 | proton acceptor, proton donor, proton relay |
| 3 | GLN166 | covalently attached, electron pair acceptor, electron pair donor, nucleofuge, nucleophile, proton acceptor |
| site_id | MCSA4 |
| Number of Residues | 2 |
| Details | M-CSA 553 |
| Chain | Residue | Details |
| 4 | ASN138 | proton acceptor, proton donor, proton relay |
| 4 | GLN166 | covalently attached, electron pair acceptor, electron pair donor, nucleofuge, nucleophile, proton acceptor |
