1NAL
THE THREE-DIMENSIONAL STRUCTURE OF N-ACETYLNEURAMINATE LYASE FROM ESCHERICHIA COLI
Experimental procedure
Spacegroup name | P 32 2 1 |
Unit cell lengths | 122.800, 122.800, 198.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.200 |
R-factor | 0.208 |
Rwork | 0.208 |
RMSD bond length | 0.015 |
RMSD bond angle | 2.010 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.200 * | 2.200 * |
Rmerge | 0.096 * | |
Total number of observations | 205465 * | |
Number of reflections | 78145 * | |
Completeness [%] | 55 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium phosphate | 75 (mM) | |
2 | 1 | reservoir | ammonium sulfate | saturated | |
3 | 1 | drop | sodium phosphate | 75 (mM) | |
4 | 1 | drop | ammonium sulfate | ||
5 | 1 | drop | protein | 17 (mg/ml) | |
6 | 1 | drop | sodium chloride | 0.1 (M) | |
7 | 1 | drop | sodium azide | 0.02 (%) | |
8 | 1 | drop | Tris-HCl | 25 (mM) |