Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1MTO

Crystal structure of a Phosphofructokinase mutant from Bacillus stearothermophilus bound with fructose-6-phosphate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003872	molecular_function	6-phosphofructokinase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005945	cellular_component	6-phosphofructokinase complex
A	0006002	biological_process	fructose 6-phosphate metabolic process
A	0006096	biological_process	glycolytic process
A	0008443	molecular_function	phosphofructokinase activity
A	0016208	molecular_function	AMP binding
A	0016301	molecular_function	kinase activity
A	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0048029	molecular_function	monosaccharide binding
A	0061621	biological_process	canonical glycolysis
A	0070095	molecular_function	fructose-6-phosphate binding
B	0003872	molecular_function	6-phosphofructokinase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005945	cellular_component	6-phosphofructokinase complex
B	0006002	biological_process	fructose 6-phosphate metabolic process
B	0006096	biological_process	glycolytic process
B	0008443	molecular_function	phosphofructokinase activity
B	0016208	molecular_function	AMP binding
B	0016301	molecular_function	kinase activity
B	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0048029	molecular_function	monosaccharide binding
B	0061621	biological_process	canonical glycolysis
B	0070095	molecular_function	fructose-6-phosphate binding
C	0003872	molecular_function	6-phosphofructokinase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005945	cellular_component	6-phosphofructokinase complex
C	0006002	biological_process	fructose 6-phosphate metabolic process
C	0006096	biological_process	glycolytic process
C	0008443	molecular_function	phosphofructokinase activity
C	0016208	molecular_function	AMP binding
C	0016301	molecular_function	kinase activity
C	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0048029	molecular_function	monosaccharide binding
C	0061621	biological_process	canonical glycolysis
C	0070095	molecular_function	fructose-6-phosphate binding
D	0003872	molecular_function	6-phosphofructokinase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005945	cellular_component	6-phosphofructokinase complex
D	0006002	biological_process	fructose 6-phosphate metabolic process
D	0006096	biological_process	glycolytic process
D	0008443	molecular_function	phosphofructokinase activity
D	0016208	molecular_function	AMP binding
D	0016301	molecular_function	kinase activity
D	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	0048029	molecular_function	monosaccharide binding
D	0061621	biological_process	canonical glycolysis
D	0070095	molecular_function	fructose-6-phosphate binding
E	0003872	molecular_function	6-phosphofructokinase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005945	cellular_component	6-phosphofructokinase complex
E	0006002	biological_process	fructose 6-phosphate metabolic process
E	0006096	biological_process	glycolytic process
E	0008443	molecular_function	phosphofructokinase activity
E	0016208	molecular_function	AMP binding
E	0016301	molecular_function	kinase activity
E	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
E	0048029	molecular_function	monosaccharide binding
E	0061621	biological_process	canonical glycolysis
E	0070095	molecular_function	fructose-6-phosphate binding
F	0003872	molecular_function	6-phosphofructokinase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005945	cellular_component	6-phosphofructokinase complex
F	0006002	biological_process	fructose 6-phosphate metabolic process
F	0006096	biological_process	glycolytic process
F	0008443	molecular_function	phosphofructokinase activity
F	0016208	molecular_function	AMP binding
F	0016301	molecular_function	kinase activity
F	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
F	0048029	molecular_function	monosaccharide binding
F	0061621	biological_process	canonical glycolysis
F	0070095	molecular_function	fructose-6-phosphate binding
G	0003872	molecular_function	6-phosphofructokinase activity
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005945	cellular_component	6-phosphofructokinase complex
G	0006002	biological_process	fructose 6-phosphate metabolic process
G	0006096	biological_process	glycolytic process
G	0008443	molecular_function	phosphofructokinase activity
G	0016208	molecular_function	AMP binding
G	0016301	molecular_function	kinase activity
G	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
G	0042802	molecular_function	identical protein binding
G	0046872	molecular_function	metal ion binding
G	0048029	molecular_function	monosaccharide binding
G	0061621	biological_process	canonical glycolysis
G	0070095	molecular_function	fructose-6-phosphate binding
H	0003872	molecular_function	6-phosphofructokinase activity
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005945	cellular_component	6-phosphofructokinase complex
H	0006002	biological_process	fructose 6-phosphate metabolic process
H	0006096	biological_process	glycolytic process
H	0008443	molecular_function	phosphofructokinase activity
H	0016208	molecular_function	AMP binding
H	0016301	molecular_function	kinase activity
H	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
H	0042802	molecular_function	identical protein binding
H	0046872	molecular_function	metal ion binding
H	0048029	molecular_function	monosaccharide binding
H	0061621	biological_process	canonical glycolysis
H	0070095	molecular_function	fructose-6-phosphate binding

Functional Information from PROSITE/UniProt

site_id	PS00433
Number of Residues	19
Details	PHOSPHOFRUCTOKINASE Phosphofructokinase signature. RvtvlGHvQRGGsptafDR

Chain	Residue	Details
A	ARG243-ARG261

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton acceptor => ECO:0000255\|HAMAP-Rule:MF_00339

Chain	Residue	Details
A	ASP127
B	ASP127
C	ASP127
D	ASP127
E	ASP127
F	ASP127
G	ASP127
H	ASP127

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00339

Chain	Residue	Details
A	GLY11
E	GLY102
F	GLY11
F	GLY102
G	GLY11
G	GLY102
H	GLY11
H	GLY102
A	GLY102
B	GLY11
B	GLY102
C	GLY11
C	GLY102
D	GLY11
D	GLY102
E	GLY11

site_id	SWS_FT_FI3
Number of Residues	40
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00339, ECO:0000269\|PubMed:6115424

Chain	Residue	Details
A	ARG21
B	ARG162
C	ARG21
C	ASP59
C	ARG72
C	ASP103
C	ARG162
D	ARG21
D	ASP59
D	ARG72
D	ASP103
A	ASP59
D	ARG162
E	ARG21
E	ASP59
E	ARG72
E	ASP103
E	ARG162
F	ARG21
F	ASP59
F	ARG72
F	ASP103
A	ARG72
F	ARG162
G	ARG21
G	ASP59
G	ARG72
G	ASP103
G	ARG162
H	ARG21
H	ASP59
H	ARG72
H	ASP103
A	ASP103
H	ARG162
A	ARG162
B	ARG21
B	ASP59
B	ARG72
B	ASP103

site_id	SWS_FT_FI4
Number of Residues	32
Details	BINDING: in other chain => ECO:0000255\|HAMAP-Rule:MF_00339, ECO:0000269\|PubMed:12390023, ECO:0000269\|PubMed:6115424

Chain	Residue	Details
A	THR125
C	MET169
C	GLU222
C	HIS249
D	THR125
D	MET169
D	GLU222
D	HIS249
E	THR125
E	MET169
E	GLU222
A	MET169
E	HIS249
F	THR125
F	MET169
F	GLU222
F	HIS249
G	THR125
G	MET169
G	GLU222
G	HIS249
H	THR125
A	GLU222
H	MET169
H	GLU222
H	HIS249
A	HIS249
B	THR125
B	MET169
B	GLU222
B	HIS249
C	THR125

site_id	SWS_FT_FI5
Number of Residues	32
Details	BINDING: in other chain => ECO:0000255\|HAMAP-Rule:MF_00339, ECO:0000269\|PubMed:6115424

Chain	Residue	Details
A	ARG154
C	GLY185
C	ARG211
C	LYS213
D	ARG154
D	GLY185
D	ARG211
D	LYS213
E	ARG154
E	GLY185
E	ARG211
A	GLY185
E	LYS213
F	ARG154
F	GLY185
F	ARG211
F	LYS213
G	ARG154
G	GLY185
G	ARG211
G	LYS213
H	ARG154
A	ARG211
H	GLY185
H	ARG211
H	LYS213
A	LYS213
B	ARG154
B	GLY185
B	ARG211
B	LYS213
C	ARG154

site_id	SWS_FT_FI6
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00339, ECO:0000269\|PubMed:12390023, ECO:0000269\|PubMed:6115424

Chain	Residue	Details
A	ARG243
B	ARG243
C	ARG243
D	ARG243
E	ARG243
F	ARG243
G	ARG243
H	ARG243

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1pfk

Chain	Residue	Details
A	GLY11
A	ASP127
A	THR125
A	ARG72
A	ARG171

site_id	CSA2
Number of Residues	5
Details	Annotated By Reference To The Literature 1pfk

Chain	Residue	Details
B	GLY11
B	ASP127
B	THR125
B	ARG72
B	ARG171

site_id	CSA3
Number of Residues	5
Details	Annotated By Reference To The Literature 1pfk

Chain	Residue	Details
C	GLY11
C	ASP127
C	THR125
C	ARG72
C	ARG171

site_id	CSA4
Number of Residues	5
Details	Annotated By Reference To The Literature 1pfk

Chain	Residue	Details
D	GLY11
D	ASP127
D	THR125
D	ARG72
D	ARG171

site_id	CSA5
Number of Residues	5
Details	Annotated By Reference To The Literature 1pfk

Chain	Residue	Details
E	GLY11
E	ASP127
E	THR125
E	ARG72
E	ARG171

site_id	CSA6
Number of Residues	5
Details	Annotated By Reference To The Literature 1pfk

Chain	Residue	Details
F	GLY11
F	ASP127
F	THR125
F	ARG72
F	ARG171

site_id	CSA7
Number of Residues	5
Details	Annotated By Reference To The Literature 1pfk

Chain	Residue	Details
G	GLY11
G	ASP127
G	THR125
G	ARG72
G	ARG171

site_id	CSA8
Number of Residues	5
Details	Annotated By Reference To The Literature 1pfk

Chain	Residue	Details
H	GLY11
H	ASP127
H	THR125
H	ARG72
H	ARG171

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)