1MTO
Crystal structure of a Phosphofructokinase mutant from Bacillus stearothermophilus bound with fructose-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-05 |
Detector | MACSCIENCE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.682, 106.872, 119.588 |
Unit cell angles | 90.00, 113.98, 90.00 |
Refinement procedure
Resolution | 29.840 - 3.100 * |
R-factor | 0.189 |
Rwork | 0.180 |
R-free | 0.27460 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pfk |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.146 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.840 | |
High resolution limit [Å] | 3.100 * | |
Rmerge | 0.101 * | |
Total number of observations | 106908 * | |
Number of reflections | 46829 | |
Completeness [%] | 91.6 * | 89.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 8000, Sodium Cacodylate, Calcium Acetate, Fructose-6-phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 12 (%) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
3 | 1 | reservoir | calcium acetate | 0.2 (M) | |
4 | 1 | reservoir | Fru-6-P | 1 (mM) | |
5 | 1 | drop | protein | 7.5 (mg/ml) |