1MPG

3-METHYLADENINE DNA GLYCOSYLASE II FROM ESCHERICHIA COLI

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0003905	molecular_function	alkylbase DNA N-glycosylase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006281	biological_process	DNA repair
A	0006284	biological_process	base-excision repair
A	0006285	biological_process	base-excision repair, AP site formation
A	0006307	biological_process	DNA alkylation repair
A	0006950	biological_process	response to stress
A	0006974	biological_process	DNA damage response
A	0008725	molecular_function	DNA-3-methyladenine glycosylase activity
A	0016787	molecular_function	hydrolase activity
A	0032131	molecular_function	alkylated DNA binding
A	0032993	cellular_component	protein-DNA complex
A	0043916	molecular_function	DNA-7-methylguanine glycosylase activity
A	0052821	molecular_function	DNA-7-methyladenine glycosylase activity
A	0052822	molecular_function	DNA-3-methylguanine glycosylase activity
B	0003824	molecular_function	catalytic activity
B	0003905	molecular_function	alkylbase DNA N-glycosylase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006281	biological_process	DNA repair
B	0006284	biological_process	base-excision repair
B	0006285	biological_process	base-excision repair, AP site formation
B	0006307	biological_process	DNA alkylation repair
B	0006950	biological_process	response to stress
B	0006974	biological_process	DNA damage response
B	0008725	molecular_function	DNA-3-methyladenine glycosylase activity
B	0016787	molecular_function	hydrolase activity
B	0032131	molecular_function	alkylated DNA binding
B	0032993	cellular_component	protein-DNA complex
B	0043916	molecular_function	DNA-7-methylguanine glycosylase activity
B	0052821	molecular_function	DNA-7-methyladenine glycosylase activity
B	0052822	molecular_function	DNA-3-methylguanine glycosylase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 283

Chain	Residue
A	GLN85
A	CYS86
A	ASN87
A	ILE90
A	TRP263
A	TYR266
A	HOH306

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site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL B 283

Chain	Residue
B	ASN87
B	ILE90
B	VAL91
B	TRP263
B	TYR266
B	HOH380
B	GLN85
B	CYS86

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Functional Information from PROSITE/UniProt

site_id	PS00516
Number of Residues	25
Details	ALKYLBASE_DNA_GLYCOS Alkylbase DNA glycosidases alkA family signature. GIGrWTAnYFaLrgwqakdvFlpDD

Chain	Residue	Details
A	GLY214-ASP238

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	ASP238
B	ASP238

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site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Determinant for substrate specificity and/or activity

Chain	Residue	Details
A	TRP218
B	TRP218

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 313

Chain	Residue	Details
A	TRP218	electrostatic stabiliser, van der waals interaction
A	TYR222	electrostatic stabiliser, van der waals interaction
A	ASP238	attractive charge-charge interaction, electrostatic stabiliser, promote heterolysis

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site_id	MCSA2
Number of Residues	3
Details	M-CSA 313

Chain	Residue	Details
B	TRP218	electrostatic stabiliser, van der waals interaction
B	TYR222	electrostatic stabiliser, van der waals interaction
B	ASP238	attractive charge-charge interaction, electrostatic stabiliser, promote heterolysis

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