1MPG
3-METHYLADENINE DNA GLYCOSYLASE II FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.080, 75.770, 60.920 |
| Unit cell angles | 90.00, 109.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.24700 |
| Structure solution method | MAD, SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.133 |
| Total number of observations | 318470 * | |
| Number of reflections | 45971 | |
| <I/σ(I)> | 20 | 12 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 7.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 4% PEG 8000, 50 MM NACL, 10 MM TRIS-CL (PH 7.5), 7.5 MM KPO4 (PH 5.9),0.1 MM EDTA, 1.5 MM DITHIOTHREITOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-5 (mg/ml) | |
| 2 | 1 | drop | 0.05 (M) | ||
| 3 | 1 | drop | Tris-Cl | 0.01 (M) | |
| 4 | 1 | drop | dithiothreitol | 1.5 (mM) | |
| 5 | 1 | drop | EDTA | 0.05 (mM) | |
| 6 | 1 | drop | potassium phosphate | 0.0075 (M) | |
| 7 | 1 | drop | PEG8000 | 3-5 (%(w/v)) | |
| 8 | 1 | reservoir | potassium phosphate | 0.015 (M) | |
| 9 | 1 | reservoir | PEG8000 | 6-10 (%(w/v)) |
