1L7D

Crystal Structure of R. rubrum Transhydrogenase Domain I without Bound NAD(H)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0006740	biological_process	NADPH regeneration
A	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
A	0016491	molecular_function	oxidoreductase activity
A	0046983	molecular_function	protein dimerization activity
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
A	0070403	molecular_function	NAD+ binding
A	0070404	molecular_function	NADH binding
A	1902600	biological_process	proton transmembrane transport
B	0000166	molecular_function	nucleotide binding
B	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0006740	biological_process	NADPH regeneration
B	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
B	0016491	molecular_function	oxidoreductase activity
B	0046983	molecular_function	protein dimerization activity
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
B	0070403	molecular_function	NAD+ binding
B	0070404	molecular_function	NADH binding
B	1902600	biological_process	proton transmembrane transport
C	0000166	molecular_function	nucleotide binding
C	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
C	0005515	molecular_function	protein binding
C	0005886	cellular_component	plasma membrane
C	0006740	biological_process	NADPH regeneration
C	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
C	0016491	molecular_function	oxidoreductase activity
C	0046983	molecular_function	protein dimerization activity
C	0050661	molecular_function	NADP binding
C	0051287	molecular_function	NAD binding
C	0070403	molecular_function	NAD+ binding
C	0070404	molecular_function	NADH binding
C	1902600	biological_process	proton transmembrane transport
D	0000166	molecular_function	nucleotide binding
D	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
D	0005515	molecular_function	protein binding
D	0005886	cellular_component	plasma membrane
D	0006740	biological_process	NADPH regeneration
D	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
D	0016491	molecular_function	oxidoreductase activity
D	0046983	molecular_function	protein dimerization activity
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
D	0070403	molecular_function	NAD+ binding
D	0070404	molecular_function	NADH binding
D	1902600	biological_process	proton transmembrane transport

Functional Information from PROSITE/UniProt

site_id	PS00836
Number of Residues	27
Details	ALADH_PNT_1 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1. AIPkErrpGEd..RVAiSPeVVkkLvGlG

Chain	Residue	Details
A	ALA4-GLY30

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site_id	PS00837
Number of Residues	26
Details	ALADH_PNT_2 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 2. VFGVGvAGlqAiatAkRLGAvVmatD

Chain	Residue	Details
A	VAL177-ASP202

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	Region: {"description":"RQD loop; involved in interaction with PntB"}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	44
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10997900","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11250201","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15670609","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details

Chain	Residue	Details
A	ASP135
A	SER138
A	ARG127
A	GLN132

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site_id	CSA2
Number of Residues	4
Details

Chain	Residue	Details
B	ARG527
B	GLN532
B	SER538
B	ASP535

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site_id	CSA3
Number of Residues	4
Details

Chain	Residue	Details
C	SER938
C	GLN932
C	ASP935
C	ARG927

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site_id	CSA4
Number of Residues	4
Details

Chain	Residue	Details
D	ASP1335
D	ARG1327
D	GLN1332
D	SER1338

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site_id	MCSA1
Number of Residues	4
Details	M-CSA 116

Chain	Residue	Details
A	ARG127	hydrogen bond donor, steric role
A	GLN132	steric locator
A	ASP135	hydrogen bond acceptor, steric role
A	SER138	electrostatic stabiliser

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site_id	MCSA2
Number of Residues	4
Details	M-CSA 116

Chain	Residue	Details
B	ARG527	hydrogen bond donor, steric role
B	GLN532	steric locator
B	ASP535	hydrogen bond acceptor, steric role
B	SER538	electrostatic stabiliser

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site_id	MCSA3
Number of Residues	4
Details	M-CSA 116

Chain	Residue	Details
C	ARG927	hydrogen bond donor, steric role
C	GLN932	steric locator
C	ASP935	hydrogen bond acceptor, steric role
C	SER938	electrostatic stabiliser

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site_id	MCSA4
Number of Residues	4
Details	M-CSA 116

Chain	Residue	Details
D	ARG1327	hydrogen bond donor, steric role
D	GLN1332	steric locator
D	ASP1335	hydrogen bond acceptor, steric role
D	SER1338	electrostatic stabiliser

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