1L7D
Crystal Structure of R. rubrum Transhydrogenase Domain I without Bound NAD(H)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-05-29 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.960, 117.130, 94.230 |
Unit cell angles | 90.00, 108.26, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.820 * |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.26200 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.270 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.076 * | 0.448 * |
Total number of observations | 209505 * | |
Number of reflections | 113060 | |
<I/σ(I)> | 8.5 | 1.9 |
Completeness [%] | 91.2 | 66.6 |
Redundancy | 1.9 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 * | 4 * | mPEG 2K, Tris-HCl, Magnesium acetate, pH 7.5, VAPOR DIFFUSION, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 22 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
3 | 1 | drop | ammonium sulfate | 10 (mM) | |
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | PMSF | 0.5 (mM) | |
6 | 1 | reservoir | PEG2000 | 18 (%(w/v)) | |
7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
8 | 1 | reservoir | magnesium acetate | 1 (mM) |