Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1KVO

HUMAN PHOSPHOLIPASE A2 COMPLEXED WITH A HIGHLY POTENT SUBSTRATE ANOLOGUE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004623	molecular_function	phospholipase A2 activity
A	0005509	molecular_function	calcium ion binding
A	0005543	molecular_function	phospholipid binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005739	cellular_component	mitochondrion
A	0005741	cellular_component	mitochondrial outer membrane
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005886	cellular_component	plasma membrane
A	0006629	biological_process	lipid metabolic process
A	0006644	biological_process	phospholipid metabolic process
A	0006954	biological_process	inflammatory response
A	0010744	biological_process	positive regulation of macrophage derived foam cell differentiation
A	0016042	biological_process	lipid catabolic process
A	0016787	molecular_function	hydrolase activity
A	0030141	cellular_component	secretory granule
A	0031640	biological_process	killing of cells of another organism
A	0034374	biological_process	low-density lipoprotein particle remodeling
A	0036335	biological_process	intestinal stem cell homeostasis
A	0042130	biological_process	negative regulation of T cell proliferation
A	0042742	biological_process	defense response to bacterium
A	0046337	biological_process	phosphatidylethanolamine metabolic process
A	0046470	biological_process	phosphatidylcholine metabolic process
A	0046473	biological_process	phosphatidic acid metabolic process
A	0046872	molecular_function	metal ion binding
A	0047498	molecular_function	calcium-dependent phospholipase A2 activity
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050482	biological_process	arachidonic acid secretion
A	0050729	biological_process	positive regulation of inflammatory response
A	0050830	biological_process	defense response to Gram-positive bacterium
A	0070062	cellular_component	extracellular exosome
A	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
A	1902563	biological_process	regulation of neutrophil activation
B	0004623	molecular_function	phospholipase A2 activity
B	0005509	molecular_function	calcium ion binding
B	0005543	molecular_function	phospholipid binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005739	cellular_component	mitochondrion
B	0005741	cellular_component	mitochondrial outer membrane
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005886	cellular_component	plasma membrane
B	0006629	biological_process	lipid metabolic process
B	0006644	biological_process	phospholipid metabolic process
B	0006954	biological_process	inflammatory response
B	0010744	biological_process	positive regulation of macrophage derived foam cell differentiation
B	0016042	biological_process	lipid catabolic process
B	0016787	molecular_function	hydrolase activity
B	0030141	cellular_component	secretory granule
B	0031640	biological_process	killing of cells of another organism
B	0034374	biological_process	low-density lipoprotein particle remodeling
B	0036335	biological_process	intestinal stem cell homeostasis
B	0042130	biological_process	negative regulation of T cell proliferation
B	0042742	biological_process	defense response to bacterium
B	0046337	biological_process	phosphatidylethanolamine metabolic process
B	0046470	biological_process	phosphatidylcholine metabolic process
B	0046473	biological_process	phosphatidic acid metabolic process
B	0046872	molecular_function	metal ion binding
B	0047498	molecular_function	calcium-dependent phospholipase A2 activity
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050482	biological_process	arachidonic acid secretion
B	0050729	biological_process	positive regulation of inflammatory response
B	0050830	biological_process	defense response to Gram-positive bacterium
B	0070062	cellular_component	extracellular exosome
B	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
B	1902563	biological_process	regulation of neutrophil activation
C	0004623	molecular_function	phospholipase A2 activity
C	0005509	molecular_function	calcium ion binding
C	0005543	molecular_function	phospholipid binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005739	cellular_component	mitochondrion
C	0005741	cellular_component	mitochondrial outer membrane
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005886	cellular_component	plasma membrane
C	0006629	biological_process	lipid metabolic process
C	0006644	biological_process	phospholipid metabolic process
C	0006954	biological_process	inflammatory response
C	0010744	biological_process	positive regulation of macrophage derived foam cell differentiation
C	0016042	biological_process	lipid catabolic process
C	0016787	molecular_function	hydrolase activity
C	0030141	cellular_component	secretory granule
C	0031640	biological_process	killing of cells of another organism
C	0034374	biological_process	low-density lipoprotein particle remodeling
C	0036335	biological_process	intestinal stem cell homeostasis
C	0042130	biological_process	negative regulation of T cell proliferation
C	0042742	biological_process	defense response to bacterium
C	0046337	biological_process	phosphatidylethanolamine metabolic process
C	0046470	biological_process	phosphatidylcholine metabolic process
C	0046473	biological_process	phosphatidic acid metabolic process
C	0046872	molecular_function	metal ion binding
C	0047498	molecular_function	calcium-dependent phospholipase A2 activity
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0050482	biological_process	arachidonic acid secretion
C	0050729	biological_process	positive regulation of inflammatory response
C	0050830	biological_process	defense response to Gram-positive bacterium
C	0070062	cellular_component	extracellular exosome
C	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
C	1902563	biological_process	regulation of neutrophil activation
D	0004623	molecular_function	phospholipase A2 activity
D	0005509	molecular_function	calcium ion binding
D	0005543	molecular_function	phospholipid binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005739	cellular_component	mitochondrion
D	0005741	cellular_component	mitochondrial outer membrane
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005886	cellular_component	plasma membrane
D	0006629	biological_process	lipid metabolic process
D	0006644	biological_process	phospholipid metabolic process
D	0006954	biological_process	inflammatory response
D	0010744	biological_process	positive regulation of macrophage derived foam cell differentiation
D	0016042	biological_process	lipid catabolic process
D	0016787	molecular_function	hydrolase activity
D	0030141	cellular_component	secretory granule
D	0031640	biological_process	killing of cells of another organism
D	0034374	biological_process	low-density lipoprotein particle remodeling
D	0036335	biological_process	intestinal stem cell homeostasis
D	0042130	biological_process	negative regulation of T cell proliferation
D	0042742	biological_process	defense response to bacterium
D	0046337	biological_process	phosphatidylethanolamine metabolic process
D	0046470	biological_process	phosphatidylcholine metabolic process
D	0046473	biological_process	phosphatidic acid metabolic process
D	0046872	molecular_function	metal ion binding
D	0047498	molecular_function	calcium-dependent phospholipase A2 activity
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0050482	biological_process	arachidonic acid secretion
D	0050729	biological_process	positive regulation of inflammatory response
D	0050830	biological_process	defense response to Gram-positive bacterium
D	0070062	cellular_component	extracellular exosome
D	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
D	1902563	biological_process	regulation of neutrophil activation
E	0004623	molecular_function	phospholipase A2 activity
E	0005509	molecular_function	calcium ion binding
E	0005543	molecular_function	phospholipid binding
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0005739	cellular_component	mitochondrion
E	0005741	cellular_component	mitochondrial outer membrane
E	0005783	cellular_component	endoplasmic reticulum
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005886	cellular_component	plasma membrane
E	0006629	biological_process	lipid metabolic process
E	0006644	biological_process	phospholipid metabolic process
E	0006954	biological_process	inflammatory response
E	0010744	biological_process	positive regulation of macrophage derived foam cell differentiation
E	0016042	biological_process	lipid catabolic process
E	0016787	molecular_function	hydrolase activity
E	0030141	cellular_component	secretory granule
E	0031640	biological_process	killing of cells of another organism
E	0034374	biological_process	low-density lipoprotein particle remodeling
E	0036335	biological_process	intestinal stem cell homeostasis
E	0042130	biological_process	negative regulation of T cell proliferation
E	0042742	biological_process	defense response to bacterium
E	0046337	biological_process	phosphatidylethanolamine metabolic process
E	0046470	biological_process	phosphatidylcholine metabolic process
E	0046473	biological_process	phosphatidic acid metabolic process
E	0046872	molecular_function	metal ion binding
E	0047498	molecular_function	calcium-dependent phospholipase A2 activity
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0050482	biological_process	arachidonic acid secretion
E	0050729	biological_process	positive regulation of inflammatory response
E	0050830	biological_process	defense response to Gram-positive bacterium
E	0070062	cellular_component	extracellular exosome
E	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
E	1902563	biological_process	regulation of neutrophil activation
F	0004623	molecular_function	phospholipase A2 activity
F	0005509	molecular_function	calcium ion binding
F	0005543	molecular_function	phospholipid binding
F	0005576	cellular_component	extracellular region
F	0005615	cellular_component	extracellular space
F	0005739	cellular_component	mitochondrion
F	0005741	cellular_component	mitochondrial outer membrane
F	0005783	cellular_component	endoplasmic reticulum
F	0005789	cellular_component	endoplasmic reticulum membrane
F	0005886	cellular_component	plasma membrane
F	0006629	biological_process	lipid metabolic process
F	0006644	biological_process	phospholipid metabolic process
F	0006954	biological_process	inflammatory response
F	0010744	biological_process	positive regulation of macrophage derived foam cell differentiation
F	0016042	biological_process	lipid catabolic process
F	0016787	molecular_function	hydrolase activity
F	0030141	cellular_component	secretory granule
F	0031640	biological_process	killing of cells of another organism
F	0034374	biological_process	low-density lipoprotein particle remodeling
F	0036335	biological_process	intestinal stem cell homeostasis
F	0042130	biological_process	negative regulation of T cell proliferation
F	0042742	biological_process	defense response to bacterium
F	0046337	biological_process	phosphatidylethanolamine metabolic process
F	0046470	biological_process	phosphatidylcholine metabolic process
F	0046473	biological_process	phosphatidic acid metabolic process
F	0046872	molecular_function	metal ion binding
F	0047498	molecular_function	calcium-dependent phospholipase A2 activity
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0050482	biological_process	arachidonic acid secretion
F	0050729	biological_process	positive regulation of inflammatory response
F	0050830	biological_process	defense response to Gram-positive bacterium
F	0070062	cellular_component	extracellular exosome
F	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
F	1902563	biological_process	regulation of neutrophil activation

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 191

Chain	Residue
A	HIS27
A	GLY29
A	GLY31
A	ASP48
A	OAP190

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 192

Chain	Residue
A	HOH1209
A	PHE23
A	GLY25
A	TYR112
A	ASN114

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA B 191

Chain	Residue
B	HIS27
B	GLY29
B	GLY31
B	ASP48
B	OAP190

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA B 192

Chain	Residue
B	PHE23
B	GLY25
B	TYR112
B	ASN114
B	HOH1242

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA C 191

Chain	Residue
C	HIS27
C	GLY29
C	GLY31
C	ASP48
C	OAP190

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA C 192

Chain	Residue
C	PHE23
C	TYR24
C	GLY25
C	TYR112
C	ASN114
C	HOH1268

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA D 191

Chain	Residue
D	HIS27
D	GLY29
D	GLY31
D	ASP48
D	OAP190

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA D 192

Chain	Residue
D	PHE23
D	GLY25
D	TYR112
D	ASN114

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA E 191

Chain	Residue
E	HIS27
E	GLY29
E	GLY31
E	ASP48
E	OAP190

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA E 192

Chain	Residue
E	PHE23
E	GLY25
E	TYR112
E	ASN114
E	HOH1334

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA F 191

Chain	Residue
F	HIS27
F	GLY29
F	GLY31
F	ASP48
F	OAP190

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA F 192

Chain	Residue
F	PHE23
F	GLY25
F	TYR112
F	ASN114
F	HOH1354
F	HOH1457

site_id	BC4
Number of Residues	13
Details	BINDING SITE FOR RESIDUE OAP A 190

Chain	Residue
A	ALA17
A	ALA18
A	TYR21
A	GLY22
A	HIS27
A	GLY29
A	VAL30
A	GLY31
A	CYS44
A	HIS47
A	ASP48
A	TYR51
A	CA191

site_id	BC5
Number of Residues	15
Details	BINDING SITE FOR RESIDUE OAP B 190

Chain	Residue
B	LEU2
B	PHE5
B	ALA17
B	ALA18
B	GLY22
B	HIS27
B	GLY29
B	VAL30
B	GLY31
B	HIS47
B	ASP48
B	TYR51
B	CA191
B	HOH1462
B	HOH1486

site_id	BC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE OAP C 190

Chain	Residue
C	ASP48
C	CA191
C	LEU2
C	PHE5
C	ILE9
C	ALA17
C	HIS27
C	GLY29
C	VAL30
C	GLY31
C	CYS44
C	HIS47

site_id	BC7
Number of Residues	12
Details	BINDING SITE FOR RESIDUE OAP D 190

Chain	Residue
D	LEU2
D	PHE5
D	GLY22
D	HIS27
D	GLY29
D	VAL30
D	GLY31
D	CYS44
D	HIS47
D	ASP48
D	TYR51
D	CA191

site_id	BC8
Number of Residues	14
Details	BINDING SITE FOR RESIDUE OAP E 190

Chain	Residue
E	LEU2
E	PHE5
E	ALA17
E	ALA18
E	GLY22
E	HIS27
E	GLY29
E	VAL30
E	GLY31
E	CYS44
E	HIS47
E	ASP48
E	TYR51
E	CA191

site_id	BC9
Number of Residues	15
Details	BINDING SITE FOR RESIDUE OAP F 190

Chain	Residue
F	LEU2
F	PHE5
F	ILE9
F	ALA17
F	TYR21
F	GLY22
F	PHE23
F	HIS27
F	GLY29
F	VAL30
F	GLY31
F	HIS47
F	ASP48
F	TYR51
F	CA191

Functional Information from PROSITE/UniProt

site_id	PS00118
Number of Residues	8
Details	PA2_HIS Phospholipase A2 histidine active site. CCVtHDcC

Chain	Residue	Details
A	CYS43-CYS50

site_id	PS00119
Number of Residues	11
Details	PA2_ASP Phospholipase A2 aspartic acid active site. LCECDKAAaTC

Chain	Residue	Details
A	LEU87-CYS97

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: ACT_SITE => ECO:0000250

Chain	Residue	Details
A	HIS47
E	ASP91
F	HIS47
F	ASP91
A	ASP91
B	HIS47
B	ASP91
C	HIS47
C	ASP91
D	HIS47
D	ASP91
E	HIS47

site_id	SWS_FT_FI2
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269\|PubMed:1948070, ECO:0000269\|PubMed:7664108, ECO:0000312\|PDB:1DB4, ECO:0000312\|PDB:1DB5, ECO:0000312\|PDB:1DCY, ECO:0000312\|PDB:1POE

Chain	Residue	Details
A	HIS27
C	GLY29
C	GLY31
C	ASP48
D	HIS27
D	GLY29
D	GLY31
D	ASP48
E	HIS27
E	GLY29
E	GLY31
A	GLY29
E	ASP48
F	HIS27
F	GLY29
F	GLY31
F	ASP48
A	GLY31
A	ASP48
B	HIS27
B	GLY29
B	GLY31
B	ASP48
C	HIS27

site_id	SWS_FT_FI3
Number of Residues	12
Details	SITE: Important for integrin binding => ECO:0000269\|PubMed:18635536

Chain	Residue	Details
A	ARG74
E	ARG100
F	ARG74
F	ARG100
A	ARG100
B	ARG74
B	ARG100
C	ARG74
C	ARG100
D	ARG74
D	ARG100
E	ARG74

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1n29

Chain	Residue	Details
A	HIS47
A	ASP91
A	GLY29

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1n29

Chain	Residue	Details
B	HIS47
B	ASP91
B	GLY29

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1n29

Chain	Residue	Details
C	HIS47
C	ASP91
C	GLY29

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1n29

Chain	Residue	Details
D	HIS47
D	ASP91
D	GLY29

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1n29

Chain	Residue	Details
E	HIS47
E	ASP91
E	GLY29

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1n29

Chain	Residue	Details
F	HIS47
F	ASP91
F	GLY29

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)