1KVO
HUMAN PHOSPHOLIPASE A2 COMPLEXED WITH A HIGHLY POTENT SUBSTRATE ANOLOGUE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | MACSCIENCE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.620, 114.440, 64.710 |
| Unit cell angles | 90.00, 119.90, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.2006 |
| Rwork | 0.201 |
| R-free | 0.27300 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.560 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.070 |
| Number of reflections | 81302 |
| Completeness [%] | 95.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 70 (%sat) | ||
| 2 | 1 | reservoir | Tris-Cl | 0.1 (M) | |
| 3 | 1 | reservoir | 0.05 (M) | ||
| 4 | 1 | drop | 35 (%sat) | ||
| 5 | 1 | drop | Tris-Cl | 0.05 (M) | |
| 6 | 1 | drop | 0.025 (M) | ||
| 7 | 1 | drop | hnps-PLA2 | 15 (mg/ml) |
