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1KPS

Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1

Functional Information from GO Data
ChainGOidnamespacecontents
A0000122biological_processnegative regulation of transcription by RNA polymerase II
A0000166molecular_functionnucleotide binding
A0000795cellular_componentsynaptonemal complex
A0001221molecular_functiontranscription coregulator binding
A0003723molecular_functionRNA binding
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005634cellular_componentnucleus
A0005635cellular_componentnuclear envelope
A0005643cellular_componentnuclear pore
A0005654cellular_componentnucleoplasm
A0005737cellular_componentcytoplasm
A0005829cellular_componentcytosol
A0006511biological_processubiquitin-dependent protein catabolic process
A0007059biological_processchromosome segregation
A0007084biological_processmitotic nuclear membrane reassembly
A0008134molecular_functiontranscription factor binding
A0016604cellular_componentnuclear body
A0016605cellular_componentPML body
A0016740molecular_functiontransferase activity
A0016925biological_processprotein sumoylation
A0019789molecular_functionSUMO transferase activity
A0019899molecular_functionenzyme binding
A0030335biological_processpositive regulation of cell migration
A0036211biological_processprotein modification process
A0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
A0043398molecular_functionHLH domain binding
A0044388molecular_functionsmall protein activating enzyme binding
A0045892biological_processnegative regulation of DNA-templated transcription
A0048471cellular_componentperinuclear region of cytoplasm
A0050804biological_processmodulation of chemical synaptic transmission
A0051168biological_processnuclear export
A0051301biological_processcell division
A0061656molecular_functionSUMO conjugating enzyme activity
A0071535molecular_functionRING-like zinc finger domain binding
A0098685cellular_componentSchaffer collateral - CA1 synapse
A0098978cellular_componentglutamatergic synapse
A0099523cellular_componentpresynaptic cytosol
A0099524cellular_componentpostsynaptic cytosol
A0106068cellular_componentSUMO ligase complex
A1990234cellular_componenttransferase complex
B0005096molecular_functionGTPase activator activity
B0007165biological_processsignal transduction
C0000122biological_processnegative regulation of transcription by RNA polymerase II
C0000166molecular_functionnucleotide binding
C0000795cellular_componentsynaptonemal complex
C0001221molecular_functiontranscription coregulator binding
C0003723molecular_functionRNA binding
C0005515molecular_functionprotein binding
C0005524molecular_functionATP binding
C0005634cellular_componentnucleus
C0005635cellular_componentnuclear envelope
C0005643cellular_componentnuclear pore
C0005654cellular_componentnucleoplasm
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0006511biological_processubiquitin-dependent protein catabolic process
C0007059biological_processchromosome segregation
C0007084biological_processmitotic nuclear membrane reassembly
C0008134molecular_functiontranscription factor binding
C0016604cellular_componentnuclear body
C0016605cellular_componentPML body
C0016740molecular_functiontransferase activity
C0016925biological_processprotein sumoylation
C0019789molecular_functionSUMO transferase activity
C0019899molecular_functionenzyme binding
C0030335biological_processpositive regulation of cell migration
C0036211biological_processprotein modification process
C0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
C0043398molecular_functionHLH domain binding
C0044388molecular_functionsmall protein activating enzyme binding
C0045892biological_processnegative regulation of DNA-templated transcription
C0048471cellular_componentperinuclear region of cytoplasm
C0050804biological_processmodulation of chemical synaptic transmission
C0051168biological_processnuclear export
C0051301biological_processcell division
C0061656molecular_functionSUMO conjugating enzyme activity
C0071535molecular_functionRING-like zinc finger domain binding
C0098685cellular_componentSchaffer collateral - CA1 synapse
C0098978cellular_componentglutamatergic synapse
C0099523cellular_componentpresynaptic cytosol
C0099524cellular_componentpostsynaptic cytosol
C0106068cellular_componentSUMO ligase complex
C1990234cellular_componenttransferase complex
D0005096molecular_functionGTPase activator activity
D0007165biological_processsignal transduction
Functional Information from PDB Data
site_idAC1
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 D 601
ChainResidue
DSER536
DLEU537
DHIS538
DHOH681

site_idAC2
Number of Residues6
DetailsBINDING SITE FOR RESIDUE SO4 C 602
ChainResidue
CHOH724
CGLY47
CLYS48
CLYS49
CGLN117
CHOH656

site_idAC3
Number of Residues3
DetailsBINDING SITE FOR RESIDUE SO4 B 603
ChainResidue
BSER536
BLEU537
BHIS538

site_idAC4
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 B 604
ChainResidue
BLYS502
BHOH660
BHOH683
DLYS502

Functional Information from PROSITE/UniProt
site_idPS00183
Number of Residues16
DetailsUBC_1 Ubiquitin-conjugating (UBC) active site signature. FHPNVyps.GtVCLsiL
ChainResidueDetails
APHE82-LEU97

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsSITE: Hydrophobic interaction with UBE2I => ECO:0000250
ChainResidueDetails
BPHE564
BLYS567
DPHE564
DLYS567

site_idSWS_FT_FI2
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P46060
ChainResidueDetails
BSER430
BSER437
DSER430
DSER437
CVAL25
CLEU57

site_idSWS_FT_FI3
Number of Residues2
DetailsMOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P46060
ChainResidueDetails
BTHR438
DTHR438

site_idSWS_FT_FI4
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079
ChainResidueDetails
BSER441
DSER441

site_idSWS_FT_FI5
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:15037602, ECO:0007744|PubMed:19131326, ECO:0007744|PubMed:21183079
ChainResidueDetails
BSER444
DSER444

site_idSWS_FT_FI6
Number of Residues2
DetailsMOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P46060
ChainResidueDetails
BLYS526
DLYS526

site_idSWS_FT_FI7
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250|UniProtKB:P46060
ChainResidueDetails
BLYS454
DLYS454

site_idSWS_FT_FI8
Number of Residues4
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0000250|UniProtKB:P46060
ChainResidueDetails
BLYS526
DLYS526

site_idSWS_FT_FI9
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733
ChainResidueDetails
ALYS48
CLYS48

site_idSWS_FT_FI10
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25114211, ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733
ChainResidueDetails
ALYS49
CLYS49

237735

PDB entries from 2025-06-18

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