1KPS

Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-07-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790, 0.9793, 0.9788, 0.9712
Spacegroup name	P 21 21 2
Unit cell lengths	86.531, 126.509, 72.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.250 - 2.500
R-factor	0.223
Rwork	0.223
R-free	0.30000
Structure solution method	3 wavelength MAD dataset collected at NSLS beamline X4A to 2.8A
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	2.660
High resolution limit [Å]	2.500	2.500
Rmerge	0.080 *	0.340 *
Total number of observations	274090 *
Number of reflections	27902 *
Completeness [%]	96.2 *	92.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	291	2.0M ammonium phosphate, 0.1M hepes, 10mM CuCl2, 5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		100 (mM)
3	1	drop	Tris-HCl	50 (mM)	pH8.0
4	1	drop	BME	1 (mM)
5	1	reservoir	lithium sulfate	1.0 (M)
6	1	reservoir	ammonium sulfate	0.5 (M)
7	1	reservoir	sodium citrate	50 (mM)	pH5.5
8	1	reservoir	glycerol	5 (%)