1KNY

KANAMYCIN NUCLEOTIDYLTRANSFERASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005524	molecular_function	ATP binding
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0046677	biological_process	response to antibiotic
B	0000166	molecular_function	nucleotide binding
B	0005524	molecular_function	ATP binding
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0046677	biological_process	response to antibiotic

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 603

Chain	Residue
A	SER39
A	ASP50
A	GLU52
A	APC556

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 604

Chain	Residue
B	SER39
B	ASP50
B	GLU52
B	APC557

---

site_id	AC3
Number of Residues	19
Details	BINDING SITE FOR RESIDUE APC A 556

Chain	Residue
A	SER39
A	ARG42
A	SER49
A	ASP50
A	GLU52
A	LEU98
A	THR99
A	GLN102
A	THR186
A	THR187
A	SER188
A	MG603
A	HOH608
A	HOH611
A	HOH631
B	GLU145
B	LYS149
B	KAN559
A	GLY38

---

site_id	AC4
Number of Residues	17
Details	BINDING SITE FOR RESIDUE APC B 557

Chain	Residue
A	GLU145
A	LYS149
A	KAN558
B	TYR37
B	GLY38
B	SER39
B	ARG42
B	SER49
B	ASP50
B	GLU52
B	LEU98
B	THR99
B	THR186
B	THR187
B	SER188
B	MG604
B	HOH619

---

site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE KAN A 558

Chain	Residue
A	SER94
A	ASP95
A	GLU141
A	GLU142
A	GLU145
B	GLU67
B	GLU76
B	APC557
B	KAN559

---

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE KAN B 559

Chain	Residue
A	GLU67
A	LYS74
A	GLU76
A	APC556
A	KAN558
B	SER94
B	ASP95
B	GLU141
B	GLU145

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	252
Details	Region: {"description":"N-terminal domain","evidences":[{"source":"PubMed","id":"7577914","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8218273","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	226
Details	Region: {"description":"C-terminal domain","evidences":[{"source":"PubMed","id":"7577914","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8218273","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"7577914","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7577914","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1KNY","evidenceCode":"ECO:0000312"},{"source":"PDB","id":"6P06","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38250052","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NML","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NMM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NMN","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21040710","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6UN8","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"6UN8","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7577914","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1KNY","evidenceCode":"ECO:0000312"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38250052","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NMK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NML","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NMN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6P01","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6P04","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6UN8","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7577914","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1KNY","evidenceCode":"ECO:0000312"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 647

Chain	Residue	Details
A	ASP50	metal ligand
A	GLU52	metal ligand
A	GLU145	activator, proton acceptor, proton donor
A	LYS149	electrostatic stabiliser, polar interaction

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 647

Chain	Residue	Details
B	ASP50	metal ligand
B	GLU52	metal ligand
B	GLU145	activator, proton acceptor, proton donor
B	LYS149	electrostatic stabiliser, polar interaction

---

site_id	CSA1
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 11258909, ChenGoodspeed1999, 7577914

Chain	Residue	Details
A	GLU145
A	LYS149

---

site_id	CSA2
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 11258909, ChenGoodspeed1999, 7577914

Chain	Residue	Details
B	GLU145
B	LYS149

---