1KNY

KANAMYCIN NUCLEOTIDYLTRANSFERASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	57.300, 102.200, 108.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	16.8 *
Rwork	0.168
RMSD bond length	0.014 *
RMSD bond angle	2.500 *
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.050 *
Total number of observations	51522 *
Number of reflections	20767 *
Completeness [%]	81.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	kanamycin A	10 (mM)
3	1	drop	PEG3350	12.5 (%)
4	1	drop		150 (mM)
5	1	drop		5 (mM)
6	1	drop	HEPES	100 (mM)
7	1	reservoir	PEG	12.5 (%)