1KFX

Crystal Structure of Human m-Calpain Form I

Functional Information from GO Data

Chain	GOid	namespace	contents
L	0000785	cellular_component	chromatin
L	0001666	biological_process	response to hypoxia
L	0001824	biological_process	blastocyst development
L	0004198	molecular_function	calcium-dependent cysteine-type endopeptidase activity
L	0005509	molecular_function	calcium ion binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005764	cellular_component	lysosome
L	0005783	cellular_component	endoplasmic reticulum
L	0005794	cellular_component	Golgi apparatus
L	0005829	cellular_component	cytosol
L	0005886	cellular_component	plasma membrane
L	0005925	cellular_component	focal adhesion
L	0006508	biological_process	proteolysis
L	0007520	biological_process	myoblast fusion
L	0007565	biological_process	female pregnancy
L	0008092	molecular_function	cytoskeletal protein binding
L	0008233	molecular_function	peptidase activity
L	0008234	molecular_function	cysteine-type peptidase activity
L	0009612	biological_process	response to mechanical stimulus
L	0009897	cellular_component	external side of plasma membrane
L	0010666	biological_process	positive regulation of cardiac muscle cell apoptotic process
L	0016020	cellular_component	membrane
L	0016540	biological_process	protein autoprocessing
L	0016787	molecular_function	hydrolase activity
L	0019899	molecular_function	enzyme binding
L	0030163	biological_process	protein catabolic process
L	0030425	cellular_component	dendrite
L	0030864	cellular_component	cortical actin cytoskeleton
L	0031143	cellular_component	pseudopodium
L	0032675	biological_process	regulation of interleukin-6 production
L	0035458	biological_process	cellular response to interferon-beta
L	0042542	biological_process	response to hydrogen peroxide
L	0042995	cellular_component	cell projection
L	0043025	cellular_component	neuronal cell body
L	0044877	molecular_function	protein-containing complex binding
L	0045121	cellular_component	membrane raft
L	0046872	molecular_function	metal ion binding
L	0048266	biological_process	behavioral response to pain
L	0051493	biological_process	regulation of cytoskeleton organization
L	0051603	biological_process	proteolysis involved in protein catabolic process
L	0070062	cellular_component	extracellular exosome
L	0071222	biological_process	cellular response to lipopolysaccharide
L	0071230	biological_process	cellular response to amino acid stimulus
L	0097038	cellular_component	perinuclear endoplasmic reticulum
L	0110158	cellular_component	calpain complex
L	1901741	biological_process	positive regulation of myoblast fusion
L	2001247	biological_process	positive regulation of phosphatidylcholine biosynthetic process
S	0005509	molecular_function	calcium ion binding

Functional Information from PROSITE/UniProt

site_id	PS00139
Number of Residues	12
Details	THIOL_PROTEASE_CYS Eukaryotic thiol (cysteine) proteases cysteine active site. QGaLGDCWLlAA

Chain	Residue	Details
L	GLN99-ALA110

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site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DSDTTGKLGfeEF

Chain	Residue	Details
S	ASP852-PHE864
S	ASP882-LEU894
L	ASP585-PHE597
L	ASP615-MET627

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q64537

Chain	Residue	Details
S	ALA809
S	ASP812
S	GLU814
S	GLU819
S	ASP837
S	ASP925

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site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448

Chain	Residue	Details
S	ASP852
L	ALA542
L	ASP545
L	GLU547
L	GLU552
L	ASP658
L	ASN661
S	ASP854
S	GLU863
S	ASP882
S	ASP884
S	GLU893
L	GLU292
L	ASP299
L	GLU323

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site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q64537, ECO:0000255|PROSITE-ProRule:PRU00448

Chain	Residue	Details
S	THR856
L	GLU626
S	LYS858
S	SER886
S	THR888
L	GLU596
L	ASP615
L	ASP617
L	SER619
L	THR621

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
S	LYS879

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 589

Chain	Residue	Details
L	GLN99	electrostatic stabiliser
L	CYS105	electrostatic stabiliser
L	HIS262	proton acceptor, proton donor
L	ASN286	electrostatic stabiliser
L	TRP288	steric role

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