1KFL
Crystal structure of phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHP synthase) from E.coli complexed with Mn2+, PEP, and Phe
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009058 | biological_process | biosynthetic process |
| A | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| A | 0009423 | biological_process | chorismate biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0042802 | molecular_function | identical protein binding |
| B | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009058 | biological_process | biosynthetic process |
| B | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| B | 0009423 | biological_process | chorismate biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0042802 | molecular_function | identical protein binding |
| C | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0009058 | biological_process | biosynthetic process |
| C | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| C | 0009423 | biological_process | chorismate biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0042802 | molecular_function | identical protein binding |
| D | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0009058 | biological_process | biosynthetic process |
| D | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| D | 0009423 | biological_process | chorismate biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0042802 | molecular_function | identical protein binding |
| E | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0008652 | biological_process | amino acid biosynthetic process |
| E | 0009058 | biological_process | biosynthetic process |
| E | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| E | 0009423 | biological_process | chorismate biosynthetic process |
| E | 0016740 | molecular_function | transferase activity |
| E | 0042802 | molecular_function | identical protein binding |
| F | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0008652 | biological_process | amino acid biosynthetic process |
| F | 0009058 | biological_process | biosynthetic process |
| F | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| F | 0009423 | biological_process | chorismate biosynthetic process |
| F | 0016740 | molecular_function | transferase activity |
| F | 0042802 | molecular_function | identical protein binding |
| G | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0008652 | biological_process | amino acid biosynthetic process |
| G | 0009058 | biological_process | biosynthetic process |
| G | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| G | 0009423 | biological_process | chorismate biosynthetic process |
| G | 0016740 | molecular_function | transferase activity |
| G | 0042802 | molecular_function | identical protein binding |
| H | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0005829 | cellular_component | cytosol |
| H | 0008652 | biological_process | amino acid biosynthetic process |
| H | 0009058 | biological_process | biosynthetic process |
| H | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| H | 0009423 | biological_process | chorismate biosynthetic process |
| H | 0016740 | molecular_function | transferase activity |
| H | 0042802 | molecular_function | identical protein binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MN A 1351 |
| Chain | Residue |
| A | CYS61 |
| A | HIS268 |
| A | GLU302 |
| A | ASP326 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN B 2351 |
| Chain | Residue |
| B | CYS61 |
| B | ARG92 |
| B | HIS268 |
| B | GLU302 |
| B | ASP326 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MN C 3351 |
| Chain | Residue |
| C | CYS61 |
| C | HIS268 |
| C | GLU302 |
| C | ASP326 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MN D 4351 |
| Chain | Residue |
| D | CYS61 |
| D | HIS268 |
| D | GLU302 |
| D | ASP326 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MN E 5351 |
| Chain | Residue |
| E | CYS61 |
| E | HIS268 |
| E | GLU302 |
| E | ASP326 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN F 6351 |
| Chain | Residue |
| F | CYS61 |
| F | ARG92 |
| F | HIS268 |
| F | GLU302 |
| F | ASP326 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MN G 7351 |
| Chain | Residue |
| G | CYS61 |
| G | HIS268 |
| G | GLU302 |
| G | ASP326 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN H 8351 |
| Chain | Residue |
| H | CYS61 |
| H | ARG92 |
| H | HIS268 |
| H | GLU302 |
| H | ASP326 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1355 |
| Chain | Residue |
| A | ARG9 |
| B | GLY225 |
| B | GLY227 |
| site_id | BC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 B 2355 |
| Chain | Residue |
| A | GLY225 |
| A | GLY227 |
| A | ASP228 |
| B | ARG9 |
| B | LYS45 |
| B | LYS48 |
| B | ASN50 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3355 |
| Chain | Residue |
| C | ARG9 |
| D | GLY225 |
| D | GLY227 |
| G | LYS45 |
| G | LYS48 |
| G | ASN50 |
| site_id | BC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 4355 |
| Chain | Residue |
| C | GLY225 |
| C | GLY227 |
| D | ARG9 |
| site_id | BC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 E 5355 |
| Chain | Residue |
| E | ARG9 |
| F | GLY225 |
| F | GLY227 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 F 6355 |
| Chain | Residue |
| E | GLY225 |
| E | GLY227 |
| F | ARG9 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 H 7355 |
| Chain | Residue |
| G | ARG9 |
| H | GLY225 |
| H | GLY227 |
| H | ASP228 |
| site_id | BC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 H 8355 |
| Chain | Residue |
| G | GLY225 |
| G | GLY227 |
| H | ARG9 |
| site_id | BC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1356 |
| Chain | Residue |
| A | THR192 |
| A | ILE193 |
| A | LYS194 |
| site_id | BC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 2356 |
| Chain | Residue |
| B | THR192 |
| B | ILE193 |
| B | LYS194 |
| site_id | CC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3356 |
| Chain | Residue |
| C | THR192 |
| C | ILE193 |
| C | LYS194 |
| site_id | CC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 4356 |
| Chain | Residue |
| D | THR192 |
| D | ILE193 |
| D | LYS194 |
| site_id | CC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 E 5356 |
| Chain | Residue |
| E | THR192 |
| E | ILE193 |
| E | LYS194 |
| site_id | CC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 F 6356 |
| Chain | Residue |
| F | THR192 |
| F | ILE193 |
| F | LYS194 |
| site_id | CC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 G 7356 |
| Chain | Residue |
| G | THR192 |
| G | ILE193 |
| G | LYS194 |
| site_id | CC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 H 8356 |
| Chain | Residue |
| H | THR192 |
| H | ILE193 |
| H | LYS194 |
| site_id | CC7 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE PHE A 1354 |
| Chain | Residue |
| A | VAL221 |
| B | ASP6 |
| B | ASP7 |
| A | MSE147 |
| A | GLN151 |
| A | ALA154 |
| A | LEU179 |
| A | SER180 |
| A | PHE209 |
| A | SER211 |
| A | LYS214 |
| site_id | CC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PHE B 2354 |
| Chain | Residue |
| A | ASP6 |
| A | ASP7 |
| B | MSE147 |
| B | GLN151 |
| B | ALA154 |
| B | GLY178 |
| B | LEU179 |
| B | SER180 |
| B | PHE209 |
| B | SER211 |
| B | LYS214 |
| B | VAL221 |
| site_id | CC9 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PHE C 3354 |
| Chain | Residue |
| C | MSE147 |
| C | PRO150 |
| C | GLN151 |
| C | ALA154 |
| C | LEU179 |
| C | SER180 |
| C | PHE209 |
| C | SER211 |
| C | LYS214 |
| C | VAL221 |
| D | ASP6 |
| D | ASP7 |
| site_id | DC1 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PHE D 4354 |
| Chain | Residue |
| C | ASP6 |
| C | ASP7 |
| D | MSE147 |
| D | PRO150 |
| D | GLN151 |
| D | ALA154 |
| D | LEU179 |
| D | SER180 |
| D | PHE209 |
| D | SER211 |
| D | LYS214 |
| D | VAL221 |
| site_id | DC2 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PHE E 5354 |
| Chain | Residue |
| E | MSE147 |
| E | PRO150 |
| E | GLN151 |
| E | ALA154 |
| E | LEU179 |
| E | SER180 |
| E | PHE209 |
| E | SER211 |
| E | LYS214 |
| E | VAL221 |
| F | ASP6 |
| F | ASP7 |
| site_id | DC3 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PHE F 6354 |
| Chain | Residue |
| E | ASP6 |
| E | ASP7 |
| F | MSE147 |
| F | GLN151 |
| F | ALA154 |
| F | GLY178 |
| F | LEU179 |
| F | SER180 |
| F | PHE209 |
| F | SER211 |
| F | LYS214 |
| F | VAL221 |
| site_id | DC4 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PHE G 7354 |
| Chain | Residue |
| G | MSE147 |
| G | PRO150 |
| G | GLN151 |
| G | ALA154 |
| G | LEU179 |
| G | SER180 |
| G | PHE209 |
| G | SER211 |
| G | LYS214 |
| G | VAL221 |
| H | ASP6 |
| H | ASP7 |
| site_id | DC5 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE PHE H 8354 |
| Chain | Residue |
| G | ASP6 |
| G | ASP7 |
| H | MSE147 |
| H | PRO150 |
| H | GLN151 |
| H | ALA154 |
| H | GLY178 |
| H | LEU179 |
| H | SER180 |
| H | PHE209 |
| H | SER211 |
| H | LYS214 |
| H | VAL221 |
| site_id | DC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PEP A 1352 |
| Chain | Residue |
| A | ARG92 |
| A | TYR94 |
| A | GLU143 |
| A | LYS186 |
| A | ARG234 |
| A | HIS268 |
| A | MSE300 |
| site_id | DC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PEP B 2352 |
| Chain | Residue |
| B | ARG92 |
| B | GLU143 |
| B | LYS186 |
| B | ARG234 |
| B | HIS268 |
| B | MSE300 |
| site_id | DC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PEP C 3352 |
| Chain | Residue |
| C | ARG92 |
| C | GLU143 |
| C | LYS186 |
| C | ARG234 |
| C | HIS268 |
| C | MSE300 |
| site_id | DC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PEP D 4352 |
| Chain | Residue |
| D | ARG92 |
| D | GLU143 |
| D | LYS186 |
| D | ARG234 |
| D | HIS268 |
| D | MSE300 |
| site_id | EC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PEP E 5352 |
| Chain | Residue |
| E | ARG92 |
| E | GLU143 |
| E | LYS186 |
| E | ARG234 |
| E | HIS268 |
| E | MSE300 |
| site_id | EC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PEP F 6352 |
| Chain | Residue |
| F | ARG92 |
| F | GLU143 |
| F | LYS186 |
| F | ARG234 |
| F | HIS268 |
| F | MSE300 |
| site_id | EC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PEP G 7352 |
| Chain | Residue |
| G | ARG92 |
| G | GLU143 |
| G | LYS186 |
| G | ARG234 |
| G | HIS268 |
| G | MSE300 |
| site_id | EC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PEP H 8352 |
| Chain | Residue |
| H | ARG92 |
| H | GLU143 |
| H | LYS186 |
| H | ARG234 |
| H | HIS268 |
| H | MSE300 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| A | HIS268 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| B | HIS268 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| C | HIS268 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| D | HIS268 |
| site_id | CSA5 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| E | HIS268 |
| site_id | CSA6 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| F | HIS268 |
| site_id | CSA7 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| G | HIS268 |
| site_id | CSA8 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| H | HIS268 |
