1K9E
Crystal structure of a mutated family-67 alpha-D-glucuronidase (E285N) from Bacillus stearothermophilus T-6, complexed with 4-O-methyl-glucuronic acid
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0033939 | molecular_function | xylan alpha-1,2-glucuronosidase activity |
| A | 0045493 | biological_process | xylan catabolic process |
| A | 0046559 | molecular_function | alpha-glucuronidase activity |
| A | 2000886 | biological_process | glucuronoxylan catabolic process |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor => ECO:0000269|PubMed:14573597 |
| Chain | Residue | Details |
| A | ASN285 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000269|PubMed:14573597 |
| Chain | Residue | Details |
| A | ASP364 | |
| A | GLU392 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 5 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | GLU158 | |
| A | LYS281 | |
| A | ARG318 | |
| A | ARG335 | |
| A | LYS359 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | ASN201 | |
| A | GLU386 | |
| A | GLU510 | |
| A | TRP540 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | SITE: Participates in a stacking interactions with the sugar rings of 4-O-MeGlcA => ECO:0000250 |
| Chain | Residue | Details |
| A | TRP150 | |
| A | TRP540 |
