1K9E
Crystal structure of a mutated family-67 alpha-D-glucuronidase (E285N) from Bacillus stearothermophilus T-6, complexed with 4-O-methyl-glucuronic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2000-07-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9766 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.576, 73.576, 330.568 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.030 - 1.850 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.21800 |
Structure solution method | AB INITIO PHASING |
Starting model (for MR) | 1k9d |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.351 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.069 | 0.234 |
Total number of observations | 354446 * | |
Number of reflections | 72670 | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 92.1 | 63.1 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | 0.1M Na-citrate pH 5.0, 14% PEG 4000, 12% Iso-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
3 | 1 | reservoir | isopropyl alcohol | 12 (%(v/v)) | |
4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.0-5.5 |