1K7W

Crystal Structure of S283A Duck Delta 2 Crystallin Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004056	molecular_function	argininosuccinate lyase activity
A	0005212	molecular_function	structural constituent of eye lens
A	0005829	cellular_component	cytosol
A	0006526	biological_process	arginine biosynthetic process
A	0016829	molecular_function	lyase activity
A	0042450	biological_process	arginine biosynthetic process via ornithine
B	0003824	molecular_function	catalytic activity
B	0004056	molecular_function	argininosuccinate lyase activity
B	0005212	molecular_function	structural constituent of eye lens
B	0005829	cellular_component	cytosol
B	0006526	biological_process	arginine biosynthetic process
B	0016829	molecular_function	lyase activity
B	0042450	biological_process	arginine biosynthetic process via ornithine
C	0003824	molecular_function	catalytic activity
C	0004056	molecular_function	argininosuccinate lyase activity
C	0005212	molecular_function	structural constituent of eye lens
C	0005829	cellular_component	cytosol
C	0006526	biological_process	arginine biosynthetic process
C	0016829	molecular_function	lyase activity
C	0042450	biological_process	arginine biosynthetic process via ornithine
D	0003824	molecular_function	catalytic activity
D	0004056	molecular_function	argininosuccinate lyase activity
D	0005212	molecular_function	structural constituent of eye lens
D	0005829	cellular_component	cytosol
D	0006526	biological_process	arginine biosynthetic process
D	0016829	molecular_function	lyase activity
D	0042450	biological_process	arginine biosynthetic process via ornithine

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE AS1 C 1001

Chain	Residue
A	THR161
C	GLN328
C	LYS331
C	HOH1023
C	HOH1070
C	HOH1086
C	HOH1123
A	HIS162
B	THR281
B	ASN291
B	HOH1131
C	SER29
C	ARG115
C	ASN116
C	TYR323

---

site_id	AC2
Number of Residues	13
Details	BINDING SITE FOR RESIDUE AS1 D 1002

Chain	Residue
B	THR161
B	HIS162
D	SER29
D	ARG115
D	ASN116
D	TYR323
D	GLN328
D	LYS331
D	HOH1024
D	HOH1037
D	HOH1151
D	HOH1152
D	HOH1153

---

site_id	AC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE AS1 B 1003

Chain	Residue
B	SER29
B	ARG115
B	ASN116
B	TYR323
B	GLN328
B	LYS331
B	HOH1007
B	HOH1059
B	HOH1077
B	HOH1092
C	THR281
C	ASN291
C	HOH1187
D	THR161
D	HIS162

---

site_id	AC4
Number of Residues	16
Details	BINDING SITE FOR RESIDUE AS1 A 1004

Chain	Residue
A	SER29
A	ARG115
A	ASN116
A	TYR323
A	GLN328
A	LYS331
A	HOH1044
A	HOH1060
A	HOH1140
A	HOH1165
A	HOH1210
A	HOH1211
C	THR161
C	HIS162
D	THR281
D	ASN291

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000305|PubMed:11698398

Chain	Residue	Details
A	HIS162
B	HIS162
C	HIS162
D	HIS162

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:11698398

Chain	Residue	Details
A	ALA283
B	ALA283
C	ALA283
D	ALA283

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: in chain A => ECO:0000269|PubMed:11698398, ECO:0007744|PDB:1K7W

Chain	Residue	Details
A	SER29
A	ASN116
B	SER29
B	ASN116
C	SER29
C	ASN116
D	SER29
D	ASN116

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: in chain C => ECO:0000269|PubMed:10029536, ECO:0000269|PubMed:11698398, ECO:0007744|PDB:1DCN, ECO:0007744|PDB:1K7W

Chain	Residue	Details
A	THR161
B	THR161
C	THR161
D	THR161

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: in chain B => ECO:0000269|PubMed:10029536, ECO:0000269|PubMed:11698398, ECO:0007744|PDB:1DCN, ECO:0007744|PDB:1K7W

Chain	Residue	Details
A	ASN291
B	ASN291
C	ASN291
D	ASN291

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	BINDING: in chain A => ECO:0000269|PubMed:10029536, ECO:0000269|PubMed:11698398, ECO:0007744|PDB:1DCN, ECO:0007744|PDB:1K7W

Chain	Residue	Details
A	TYR323
A	LYS331
B	TYR323
B	LYS331
C	TYR323
C	LYS331
D	TYR323
D	LYS331

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	BINDING: in chain A => ECO:0000269|PubMed:10029536, ECO:0007744|PDB:1DCN

Chain	Residue	Details
A	GLN328
B	GLN328
C	GLN328
D	GLN328

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	SITE: Increases basicity of active site His => ECO:0000305|PubMed:11698398

Chain	Residue	Details
A	GLU296
B	GLU296
C	GLU296
D	GLU296

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1auw

Chain	Residue	Details
A	THR161
A	HIS162
B	GLU296
B	ALA283
B	LYS289

---

site_id	CSA2
Number of Residues	5
Details	Annotated By Reference To The Literature 1auw

Chain	Residue	Details
A	GLU296
A	ALA283
A	LYS289
B	THR161
B	HIS162

---

site_id	CSA3
Number of Residues	5
Details	Annotated By Reference To The Literature 1auw

Chain	Residue	Details
C	THR161
C	HIS162
D	GLU296
D	ALA283
D	LYS289

---

site_id	CSA4
Number of Residues	5
Details	Annotated By Reference To The Literature 1auw

Chain	Residue	Details
C	GLU296
C	ALA283
C	LYS289
D	THR161
D	HIS162

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 408

Chain	Residue	Details
A	HIS162	proton shuttle (general acid/base)
A	ALA283	proton shuttle (general acid/base)
A	LYS289	electrostatic stabiliser
A	GLU296	activator, proton shuttle (general acid/base)

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 408

Chain	Residue	Details
B	HIS162	proton shuttle (general acid/base)
B	ALA283	proton shuttle (general acid/base)
B	LYS289	electrostatic stabiliser
B	GLU296	activator, proton shuttle (general acid/base)

---

site_id	MCSA3
Number of Residues	4
Details	M-CSA 408

Chain	Residue	Details
C	HIS162	proton shuttle (general acid/base)
C	ALA283	proton shuttle (general acid/base)
C	LYS289	electrostatic stabiliser
C	GLU296	activator, proton shuttle (general acid/base)

---

site_id	MCSA4
Number of Residues	4
Details	M-CSA 408

Chain	Residue	Details
D	HIS162	proton shuttle (general acid/base)
D	ALA283	proton shuttle (general acid/base)
D	LYS289	electrostatic stabiliser
D	GLU296	activator, proton shuttle (general acid/base)

---