1K7W
Crystal Structure of S283A Duck Delta 2 Crystallin Mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004056 | molecular_function | argininosuccinate lyase activity |
| A | 0005212 | molecular_function | structural constituent of eye lens |
| A | 0005829 | cellular_component | cytosol |
| A | 0006526 | biological_process | L-arginine biosynthetic process |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0042450 | biological_process | L-arginine biosynthetic process via ornithine |
| A | 0170033 | biological_process | L-amino acid metabolic process |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004056 | molecular_function | argininosuccinate lyase activity |
| B | 0005212 | molecular_function | structural constituent of eye lens |
| B | 0005829 | cellular_component | cytosol |
| B | 0006526 | biological_process | L-arginine biosynthetic process |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0042450 | biological_process | L-arginine biosynthetic process via ornithine |
| B | 0170033 | biological_process | L-amino acid metabolic process |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004056 | molecular_function | argininosuccinate lyase activity |
| C | 0005212 | molecular_function | structural constituent of eye lens |
| C | 0005829 | cellular_component | cytosol |
| C | 0006526 | biological_process | L-arginine biosynthetic process |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0016829 | molecular_function | lyase activity |
| C | 0042450 | biological_process | L-arginine biosynthetic process via ornithine |
| C | 0170033 | biological_process | L-amino acid metabolic process |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004056 | molecular_function | argininosuccinate lyase activity |
| D | 0005212 | molecular_function | structural constituent of eye lens |
| D | 0005829 | cellular_component | cytosol |
| D | 0006526 | biological_process | L-arginine biosynthetic process |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0016829 | molecular_function | lyase activity |
| D | 0042450 | biological_process | L-arginine biosynthetic process via ornithine |
| D | 0170033 | biological_process | L-amino acid metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE AS1 C 1001 |
| Chain | Residue |
| A | THR161 |
| C | GLN328 |
| C | LYS331 |
| C | HOH1023 |
| C | HOH1070 |
| C | HOH1086 |
| C | HOH1123 |
| A | HIS162 |
| B | THR281 |
| B | ASN291 |
| B | HOH1131 |
| C | SER29 |
| C | ARG115 |
| C | ASN116 |
| C | TYR323 |
| site_id | AC2 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE AS1 D 1002 |
| Chain | Residue |
| B | THR161 |
| B | HIS162 |
| D | SER29 |
| D | ARG115 |
| D | ASN116 |
| D | TYR323 |
| D | GLN328 |
| D | LYS331 |
| D | HOH1024 |
| D | HOH1037 |
| D | HOH1151 |
| D | HOH1152 |
| D | HOH1153 |
| site_id | AC3 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE AS1 B 1003 |
| Chain | Residue |
| B | SER29 |
| B | ARG115 |
| B | ASN116 |
| B | TYR323 |
| B | GLN328 |
| B | LYS331 |
| B | HOH1007 |
| B | HOH1059 |
| B | HOH1077 |
| B | HOH1092 |
| C | THR281 |
| C | ASN291 |
| C | HOH1187 |
| D | THR161 |
| D | HIS162 |
| site_id | AC4 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE AS1 A 1004 |
| Chain | Residue |
| A | SER29 |
| A | ARG115 |
| A | ASN116 |
| A | TYR323 |
| A | GLN328 |
| A | LYS331 |
| A | HOH1044 |
| A | HOH1060 |
| A | HOH1140 |
| A | HOH1165 |
| A | HOH1210 |
| A | HOH1211 |
| C | THR161 |
| C | HIS162 |
| D | THR281 |
| D | ASN291 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"11698398","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"11698398","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | Binding site: {"description":"in chain A","evidences":[{"source":"PubMed","id":"11698398","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1K7W","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Binding site: {"description":"in chain C","evidences":[{"source":"PubMed","id":"10029536","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11698398","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1DCN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K7W","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Binding site: {"description":"in chain B","evidences":[{"source":"PubMed","id":"10029536","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11698398","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1DCN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K7W","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | Binding site: {"description":"in chain A","evidences":[{"source":"PubMed","id":"10029536","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11698398","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1DCN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K7W","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | Binding site: {"description":"in chain A","evidences":[{"source":"PubMed","id":"10029536","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1DCN","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | Site: {"description":"Increases basicity of active site His","evidences":[{"source":"PubMed","id":"11698398","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1auw |
| Chain | Residue | Details |
| A | THR161 | |
| A | HIS162 | |
| B | GLU296 | |
| B | ALA283 | |
| B | LYS289 |
| site_id | CSA2 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1auw |
| Chain | Residue | Details |
| A | GLU296 | |
| A | ALA283 | |
| A | LYS289 | |
| B | THR161 | |
| B | HIS162 |
| site_id | CSA3 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1auw |
| Chain | Residue | Details |
| C | THR161 | |
| C | HIS162 | |
| D | GLU296 | |
| D | ALA283 | |
| D | LYS289 |
| site_id | CSA4 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1auw |
| Chain | Residue | Details |
| C | GLU296 | |
| C | ALA283 | |
| C | LYS289 | |
| D | THR161 | |
| D | HIS162 |
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 408 |
| Chain | Residue | Details |
| A | HIS162 | proton shuttle (general acid/base) |
| A | ALA283 | proton shuttle (general acid/base) |
| A | LYS289 | electrostatic stabiliser |
| A | GLU296 | activator, proton shuttle (general acid/base) |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 408 |
| Chain | Residue | Details |
| B | HIS162 | proton shuttle (general acid/base) |
| B | ALA283 | proton shuttle (general acid/base) |
| B | LYS289 | electrostatic stabiliser |
| B | GLU296 | activator, proton shuttle (general acid/base) |
| site_id | MCSA3 |
| Number of Residues | 4 |
| Details | M-CSA 408 |
| Chain | Residue | Details |
| C | HIS162 | proton shuttle (general acid/base) |
| C | ALA283 | proton shuttle (general acid/base) |
| C | LYS289 | electrostatic stabiliser |
| C | GLU296 | activator, proton shuttle (general acid/base) |
| site_id | MCSA4 |
| Number of Residues | 4 |
| Details | M-CSA 408 |
| Chain | Residue | Details |
| D | HIS162 | proton shuttle (general acid/base) |
| D | ALA283 | proton shuttle (general acid/base) |
| D | LYS289 | electrostatic stabiliser |
| D | GLU296 | activator, proton shuttle (general acid/base) |
