1K7W
Crystal Structure of S283A Duck Delta 2 Crystallin Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.759, 98.637, 106.147 |
| Unit cell angles | 90.00, 101.30, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.960 |
| R-factor | 0.207 * |
| Rwork | 0.207 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hy1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 19.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 1.940 * | 1.960 |
| Rmerge | 0.080 * | 0.340 * |
| Total number of observations | 692205 * | |
| Number of reflections | 127416 * | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 94.0 | 91.8 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | 12% PEG 2000 MME, 300 mM magnesium chloride, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | argininosuccinate | 75 (mM) | |
| 5 | 1 | reservoir | PEG2000MME | 12 (%(w/v)) | |
| 6 | 1 | reservoir | 300 (mM) | ||
| 7 | 1 | reservoir | HEPES | 100 (mM) | pH7.4 |
