1K4T
HUMAN DNA TOPOISOMERASE I (70 KDA) IN COMPLEX WITH THE POISON TOPOTECAN AND COVALENT COMPLEX WITH A 22 BASE PAIR DNA DUPLEX
Functional Information from GO Data
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE TTC D 990 |
| Chain | Residue |
| A | ARG364 |
| D | DC112 |
| D | DA113 |
| D | HOH1002 |
| A | LYS532 |
| A | ASP533 |
| A | THR718 |
| B | DT10 |
| D | HOH3 |
| C | TGP11 |
| C | HOH28 |
| C | HOH133 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE HG A 900 |
| Chain | Residue |
| A | ARG590 |
| A | LEU629 |
| A | CYS630 |
| site_id | AC3 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE TTG C 991 |
| Chain | Residue |
| A | ARG364 |
| A | LYS532 |
| A | ASP533 |
| A | THR718 |
| A | ASN722 |
| A | PTR723 |
| B | DT10 |
| D | HOH3 |
| C | TGP11 |
| C | HOH28 |
| C | HOH133 |
| D | DC112 |
| D | DA113 |
| D | HOH997 |
| D | HOH1002 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PG4 A 901 |
| Chain | Residue |
| A | ASP500 |
| A | GLU510 |
| A | HIS511 |
| A | LEU530 |
Functional Information from PROSITE/UniProt
| site_id | PS00176 |
| Number of Residues | 19 |
| Details | TOPO_IB_1 Topoisomerase (Topo) IB-type active site signature. EnkqialGTSKlnYLdprI |
| Chain | Residue | Details |
| A | GLU710-ILE728 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Region: {"description":"Interaction with DNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"description":"O-(3'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU01382","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10130","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10841763","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12426403","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12533542","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15165849","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15801827","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16033260","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9488644","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 9 |
| Details | Site: {"description":"Interaction with DNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by CK2","evidences":[{"source":"PubMed","id":"23185622","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25755297","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 5 |
| Details | a catalytic site defined by CSA, PubMed 9488652, 14594810 |
| Chain | Residue | Details |
| A | TYR723 | |
| A | ARG488 | |
| A | ARG590 | |
| A | HIS632 | |
| A | LYS532 |
