1JD9

CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT K300Q OF PSEUDOALTEROMONAS HALOPLANCTIS ALPHA-AMYLASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004556	molecular_function	alpha-amylase activity
A	0005975	biological_process	carbohydrate metabolic process
A	0043169	molecular_function	cation binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 800

Chain	Residue
A	ASN88
A	GLN135
A	ASP144
A	HIS178
A	HOH1117
A	HOH1118
A	HOH1437

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:11914073

Chain	Residue	Details
A	ASP174

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000269|PubMed:11914073

Chain	Residue	Details
A	GLU200

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site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000305|PubMed:11914073

Chain	Residue	Details
A	TRP47
A	HIS89
A	LYS177
A	GLU207
A	ASP264
A	HIS269

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site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:11914073, ECO:0000269|PubMed:12021442, ECO:0000269|PubMed:9541387, ECO:0007744|PDB:1AQH

Chain	Residue	Details
A	ASN88
A	GLN135
A	ASP144
A	HIS178

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ARG172

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site_id	SWS_FT_FI6
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:9541387, ECO:0007744|PDB:1AQH

Chain	Residue	Details
A	ASN262

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site_id	SWS_FT_FI7
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11914073, ECO:0000269|PubMed:9541387, ECO:0007744|PDB:1AQH

Chain	Residue	Details
A	GLN300

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site_id	SWS_FT_FI8
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000250

Chain	Residue	Details
A	ASP264

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	GLN199
A	ASP174

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site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP264
A	GLU200
A	ASP174

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	GLU200
A	ASP174

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site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP174
A	ASP203

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site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP264
A	GLU207
A	ASP174

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