1JD9
CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT K300Q OF PSEUDOALTEROMONAS HALOPLANCTIS ALPHA-AMYLASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-29 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.500, 138.400, 115.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.700 - 2.500 |
Rwork | 0.180 |
R-free | 0.24500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1aqh |
RMSD bond length | 0.007 |
RMSD bond angle | 1.267 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.030 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.216 | 0.564 |
Number of reflections | 16759 | |
<I/σ(I)> | 3 | |
Completeness [%] | 81.8 | 39.2 |
Redundancy | 3.4 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | MPD, Hepes, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 292K |