1JCH
Crystal Structure of Colicin E3 in Complex with its Immunity Protein
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000049 | molecular_function | tRNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005727 | cellular_component | extrachromosomal circular DNA |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016788 | molecular_function | hydrolase activity, acting on ester bonds |
| A | 0016829 | molecular_function | lyase activity |
| A | 0019843 | molecular_function | rRNA binding |
| A | 0031640 | biological_process | killing of cells of another organism |
| A | 0032413 | biological_process | negative regulation of ion transmembrane transporter activity |
| A | 0042742 | biological_process | defense response to bacterium |
| A | 0043022 | molecular_function | ribosome binding |
| A | 0044325 | molecular_function | transmembrane transporter binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0015643 | molecular_function | toxic substance binding |
| B | 0030153 | biological_process | bacteriocin immunity |
| C | 0000049 | molecular_function | tRNA binding |
| C | 0003723 | molecular_function | RNA binding |
| C | 0004518 | molecular_function | nuclease activity |
| C | 0004519 | molecular_function | endonuclease activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005576 | cellular_component | extracellular region |
| C | 0005727 | cellular_component | extrachromosomal circular DNA |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0016788 | molecular_function | hydrolase activity, acting on ester bonds |
| C | 0016829 | molecular_function | lyase activity |
| C | 0019843 | molecular_function | rRNA binding |
| C | 0031640 | biological_process | killing of cells of another organism |
| C | 0032413 | biological_process | negative regulation of ion transmembrane transporter activity |
| C | 0042742 | biological_process | defense response to bacterium |
| C | 0043022 | molecular_function | ribosome binding |
| C | 0044325 | molecular_function | transmembrane transporter binding |
| D | 0005515 | molecular_function | protein binding |
| D | 0015643 | molecular_function | toxic substance binding |
| D | 0030153 | biological_process | bacteriocin immunity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CIT A 601 |
| Chain | Residue |
| A | ARG545 |
| A | ASN546 |
| A | ILE547 |
| A | LYS549 |
| A | HOH925 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CIT A 602 |
| Chain | Residue |
| A | HOH5980 |
| A | GLN512 |
| A | HIS513 |
| A | HOH922 |
| A | HOH983 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CIT C 5601 |
| Chain | Residue |
| C | ARG545 |
| C | ASN546 |
| C | ILE547 |
| C | LYS549 |
| C | HOH917 |
| C | HOH5925 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CIT C 5602 |
| Chain | Residue |
| C | GLN512 |
| C | HIS513 |
| C | HOH980 |
| C | HOH5922 |
| C | HOH5983 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL C 701 |
| Chain | Residue |
| A | PHE378 |
| C | VAL139 |
| C | LEU140 |
| C | ASP187 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL B 702 |
| Chain | Residue |
| A | LYS458 |
| B | GLY1 |
| B | ASP27 |
| B | TYR79 |
| B | ASP81 |
| B | GLY82 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A 5701 |
| Chain | Residue |
| A | VAL139 |
| A | LEU140 |
| A | ASP187 |
| C | PHE378 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL D 5702 |
| Chain | Residue |
| C | LYS458 |
| D | GLY1 |
| D | ASP27 |
| D | TYR79 |
| D | ASP81 |
| D | GLY82 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000305|PubMed:11741540, ECO:0000305|PubMed:20852642 |
| Chain | Residue | Details |
| A | HIS513 | |
| C | HIS513 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000305|PubMed:11741540, ECO:0000305|PubMed:20852642 |
| Chain | Residue | Details |
| A | GLU517 | |
| C | GLU517 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000305|PubMed:11741540 |
| Chain | Residue | Details |
| A | ARG545 | |
| C | ARG545 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | SITE: Stabilizes positive charge on His-513 => ECO:0000305|PubMed:20852642 |
| Chain | Residue | Details |
| A | ASP510 | |
| C | ASP510 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 530 |
| Chain | Residue | Details |
| A | ASP510 | electrostatic stabiliser, increase acidity |
| A | HIS513 | promote heterolysis, proton acceptor, proton donor |
| A | GLU517 | increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| A | ARG545 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 530 |
| Chain | Residue | Details |
| C | ASP510 | electrostatic stabiliser, increase acidity |
| C | HIS513 | promote heterolysis, proton acceptor, proton donor |
| C | GLU517 | increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| C | ARG545 | electrostatic stabiliser |
