1JCH

Crystal Structure of Colicin E3 in Complex with its Immunity Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-19
Detector	ADSC QUANTUM 4
Wavelength(s)	1.037
Spacegroup name	P 1 21 1
Unit cell lengths	66.986, 195.733, 85.124
Unit cell angles	90.00, 113.22, 90.00

Refinement procedure

Resolution	20.170 - 3.020
R-factor	0.233 *
Rwork	0.237
R-free	0.28200 *
Structure solution method	MIR
RMSD bond length	0.015
RMSD bond angle	24.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	3.020	3.000
Rmerge	0.067	0.197
Number of reflections	37014
<I/σ(I)>	19.5	3.8
Completeness [%]	94.1	86.4
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	4 *	SODIUM CITRATE, CADMIUM ACETATE, ph 5.6, VAPOR DIFFUSION, HANGING DROP at 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	1 (M)	pH5.6