Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1IGW

Crystal Structure of the Isocitrate Lyase from the A219C mutant of Escherichia coli

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004451	molecular_function	isocitrate lyase activity
A	0005829	cellular_component	cytosol
A	0006097	biological_process	glyoxylate cycle
A	0006099	biological_process	tricarboxylic acid cycle
A	0016829	molecular_function	lyase activity
A	0019752	biological_process	carboxylic acid metabolic process
A	0043169	molecular_function	cation binding
A	0046872	molecular_function	metal ion binding
B	0003824	molecular_function	catalytic activity
B	0004451	molecular_function	isocitrate lyase activity
B	0005829	cellular_component	cytosol
B	0006097	biological_process	glyoxylate cycle
B	0006099	biological_process	tricarboxylic acid cycle
B	0016829	molecular_function	lyase activity
B	0019752	biological_process	carboxylic acid metabolic process
B	0043169	molecular_function	cation binding
B	0046872	molecular_function	metal ion binding
C	0003824	molecular_function	catalytic activity
C	0004451	molecular_function	isocitrate lyase activity
C	0005829	cellular_component	cytosol
C	0006097	biological_process	glyoxylate cycle
C	0006099	biological_process	tricarboxylic acid cycle
C	0016829	molecular_function	lyase activity
C	0019752	biological_process	carboxylic acid metabolic process
C	0043169	molecular_function	cation binding
C	0046872	molecular_function	metal ion binding
D	0003824	molecular_function	catalytic activity
D	0004451	molecular_function	isocitrate lyase activity
D	0005829	cellular_component	cytosol
D	0006097	biological_process	glyoxylate cycle
D	0006099	biological_process	tricarboxylic acid cycle
D	0016829	molecular_function	lyase activity
D	0019752	biological_process	carboxylic acid metabolic process
D	0043169	molecular_function	cation binding
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE HG A 435

Chain	Residue
A	GLU42
A	CYS43
A	HOH1445
A	HOH1600

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE HG A 436

Chain	Residue
A	LYS173
A	CYS219
A	THR223
C	HOH1514
C	HOH1571

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HG A 437

Chain	Residue
A	CYS245
A	ASP246
B	HOH1609

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HG A 438

Chain	Residue
A	CYS288
A	THR290
A	HOH1561

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE HG A 439

Chain	Residue
A	CYS318
A	HOH1461
A	HOH1507
A	HOH1549
D	MET375

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HG B 435

Chain	Residue
B	GLU42
B	CYS43
B	HOH1509

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE HG B 436

Chain	Residue
B	CYS219
B	THR223

site_id	AC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE HG B 437

Chain	Residue
B	CYS245

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HG B 438

Chain	Residue
B	CYS288
B	THR290
B	HOH1570

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE HG B 439

Chain	Residue
B	CYS318
B	PHE349
B	HOH1512
C	MET375

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE HG C 435

Chain	Residue
C	GLU42
C	CYS43

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HG C 436

Chain	Residue
A	HOH1596
C	CYS219
C	THR223

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE HG C 437

Chain	Residue
C	CYS245
C	ASP246
C	HOH1652
D	GLU260

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE HG C 438

Chain	Residue
C	CYS288
C	THR290
C	HOH1516
C	HOH1640

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE HG C 439

Chain	Residue
B	MET375
C	CYS318
C	PHE349
C	HOH1510
C	HOH1561

site_id	BC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE HG C 440

Chain	Residue
C	CYS195
C	HIS197

site_id	BC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE HG D 435

Chain	Residue
D	GLU42
D	CYS43

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HG D 436

Chain	Residue
D	LYS173
D	CYS219
D	THR223

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE HG D 437

Chain	Residue
C	GLU260
C	HOH1688
D	CYS245
D	ASP246

site_id	CC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE HG D 438

Chain	Residue
D	CYS288
D	THR290

site_id	CC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE HG D 439

Chain	Residue
D	CYS318
D	GLN337
D	PHE349
D	HOH1562

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 441

Chain	Residue
A	ASP157
A	PYR1444
A	HOH1630
A	HOH1631
A	HOH1632

site_id	CC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 441

Chain	Residue
B	ASP157
B	PYR1445
B	HOH1633
B	HOH1634
B	HOH1635

site_id	CC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 441

Chain	Residue
C	ASP157
C	PYR1446
C	HOH1694
C	HOH1695
C	HOH1696

site_id	CC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D 441

Chain	Residue
D	ASP157
D	PYR1447
D	HOH1584
D	HOH1585
D	HOH1586

site_id	CC8
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PYR A 1444

Chain	Residue
A	HOH1631
A	HOH1632
A	TYR89
A	SER91
A	GLY92
A	TRP93
A	ASP157
A	HIS184
A	ARG232
A	THR351
A	MG441
A	HOH1570
A	HOH1630

site_id	CC9
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PYR B 1445

Chain	Residue
B	TYR89
B	SER91
B	GLY92
B	TRP93
B	ASP157
B	HIS184
B	ARG232
B	THR351
B	MG441
B	HOH1633
B	HOH1635

site_id	DC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PYR C 1446

Chain	Residue
C	TYR89
C	SER91
C	GLY92
C	TRP93
C	ASP157
C	HIS184
C	ARG232
C	THR351
C	MG441
C	HOH1552
C	HOH1635
C	HOH1695
C	HOH1696

site_id	DC2
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PYR D 1447

Chain	Residue
D	TYR89
D	SER91
D	GLY92
D	TRP93
D	ASP157
D	HIS184
D	ARG232
D	THR351
D	MG441
D	HOH1552
D	HOH1584
D	HOH1585
D	HOH1586

Functional Information from PROSITE/UniProt

site_id	PS00161
Number of Residues	6
Details	ISOCITRATE_LYASE Isocitrate lyase signature. KKCGHM

Chain	Residue	Details
A	LYS193-MET198

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000269\|PubMed:7826335

Chain	Residue	Details
A	CYS195
B	CYS195
C	CYS195
D	CYS195

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:11526312

Chain	Residue	Details
A	SER91
D	SER91
D	ASP157
D	ARG232
A	ASP157
A	ARG232
B	SER91
B	ASP157
B	ARG232
C	SER91
C	ASP157
C	ARG232

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P9WKK7

Chain	Residue	Details
A	GLY196
D	GLY196
D	ASN317
D	THR351
A	ASN317
A	THR351
B	GLY196
B	ASN317
B	THR351
C	GLY196
C	ASN317
C	THR351

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1f8m

Chain	Residue	Details
A	CYS195
A	HIS184
A	ARG232

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1f8m

Chain	Residue	Details
B	HIS184
B	ARG232

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1f8m

Chain	Residue	Details
C	CYS195
C	HIS184
C	ARG232

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1f8m

Chain	Residue	Details
D	HIS184
D	ARG232

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)