Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1IB1

CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERASE COMPLEX

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0001525	biological_process	angiogenesis
A	0003016	biological_process	respiratory system process
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005925	cellular_component	focal adhesion
A	0006468	biological_process	protein phosphorylation
A	0006605	biological_process	protein targeting
A	0007165	biological_process	signal transduction
A	0008039	biological_process	synaptic target recognition
A	0008104	biological_process	protein localization
A	0019901	molecular_function	protein kinase binding
A	0019904	molecular_function	protein domain specific binding
A	0030324	biological_process	lung development
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0031647	biological_process	regulation of protein stability
A	0031982	cellular_component	vesicle
A	0035148	biological_process	tube formation
A	0042149	biological_process	cellular response to glucose starvation
A	0042470	cellular_component	melanosome
A	0042802	molecular_function	identical protein binding
A	0043066	biological_process	negative regulation of apoptotic process
A	0043067	biological_process	regulation of programmed cell death
A	0044325	molecular_function	transmembrane transporter binding
A	0045296	molecular_function	cadherin binding
A	0045824	biological_process	negative regulation of innate immune response
A	0050815	molecular_function	phosphoserine residue binding
A	0051683	biological_process	establishment of Golgi localization
A	0070062	cellular_component	extracellular exosome
A	0070371	biological_process	ERK1 and ERK2 cascade
A	0070372	biological_process	regulation of ERK1 and ERK2 cascade
A	0072562	cellular_component	blood microparticle
A	0090128	biological_process	regulation of synapse maturation
A	0090168	biological_process	Golgi reassembly
A	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
A	0098978	cellular_component	glutamatergic synapse
A	0140297	molecular_function	DNA-binding transcription factor binding
A	0140311	molecular_function	protein sequestering activity
A	1900181	biological_process	negative regulation of protein localization to nucleus
A	1904262	biological_process	negative regulation of TORC1 signaling
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0001525	biological_process	angiogenesis
B	0003016	biological_process	respiratory system process
B	0003723	molecular_function	RNA binding
B	0005515	molecular_function	protein binding
B	0005615	cellular_component	extracellular space
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005925	cellular_component	focal adhesion
B	0006468	biological_process	protein phosphorylation
B	0006605	biological_process	protein targeting
B	0007165	biological_process	signal transduction
B	0008039	biological_process	synaptic target recognition
B	0008104	biological_process	protein localization
B	0019901	molecular_function	protein kinase binding
B	0019904	molecular_function	protein domain specific binding
B	0030324	biological_process	lung development
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0031647	biological_process	regulation of protein stability
B	0031982	cellular_component	vesicle
B	0035148	biological_process	tube formation
B	0042149	biological_process	cellular response to glucose starvation
B	0042470	cellular_component	melanosome
B	0042802	molecular_function	identical protein binding
B	0043066	biological_process	negative regulation of apoptotic process
B	0043067	biological_process	regulation of programmed cell death
B	0044325	molecular_function	transmembrane transporter binding
B	0045296	molecular_function	cadherin binding
B	0045824	biological_process	negative regulation of innate immune response
B	0050815	molecular_function	phosphoserine residue binding
B	0051683	biological_process	establishment of Golgi localization
B	0070062	cellular_component	extracellular exosome
B	0070371	biological_process	ERK1 and ERK2 cascade
B	0070372	biological_process	regulation of ERK1 and ERK2 cascade
B	0072562	cellular_component	blood microparticle
B	0090128	biological_process	regulation of synapse maturation
B	0090168	biological_process	Golgi reassembly
B	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
B	0098978	cellular_component	glutamatergic synapse
B	0140297	molecular_function	DNA-binding transcription factor binding
B	0140311	molecular_function	protein sequestering activity
B	1900181	biological_process	negative regulation of protein localization to nucleus
B	1904262	biological_process	negative regulation of TORC1 signaling
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0001525	biological_process	angiogenesis
C	0003016	biological_process	respiratory system process
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005615	cellular_component	extracellular space
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005925	cellular_component	focal adhesion
C	0006468	biological_process	protein phosphorylation
C	0006605	biological_process	protein targeting
C	0007165	biological_process	signal transduction
C	0008039	biological_process	synaptic target recognition
C	0008104	biological_process	protein localization
C	0019901	molecular_function	protein kinase binding
C	0019904	molecular_function	protein domain specific binding
C	0030324	biological_process	lung development
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0031647	biological_process	regulation of protein stability
C	0031982	cellular_component	vesicle
C	0035148	biological_process	tube formation
C	0042149	biological_process	cellular response to glucose starvation
C	0042470	cellular_component	melanosome
C	0042802	molecular_function	identical protein binding
C	0043066	biological_process	negative regulation of apoptotic process
C	0043067	biological_process	regulation of programmed cell death
C	0044325	molecular_function	transmembrane transporter binding
C	0045296	molecular_function	cadherin binding
C	0045824	biological_process	negative regulation of innate immune response
C	0050815	molecular_function	phosphoserine residue binding
C	0051683	biological_process	establishment of Golgi localization
C	0070062	cellular_component	extracellular exosome
C	0070371	biological_process	ERK1 and ERK2 cascade
C	0070372	biological_process	regulation of ERK1 and ERK2 cascade
C	0072562	cellular_component	blood microparticle
C	0090128	biological_process	regulation of synapse maturation
C	0090168	biological_process	Golgi reassembly
C	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
C	0098978	cellular_component	glutamatergic synapse
C	0140297	molecular_function	DNA-binding transcription factor binding
C	0140311	molecular_function	protein sequestering activity
C	1900181	biological_process	negative regulation of protein localization to nucleus
C	1904262	biological_process	negative regulation of TORC1 signaling
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0001525	biological_process	angiogenesis
D	0003016	biological_process	respiratory system process
D	0003723	molecular_function	RNA binding
D	0005515	molecular_function	protein binding
D	0005615	cellular_component	extracellular space
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005925	cellular_component	focal adhesion
D	0006468	biological_process	protein phosphorylation
D	0006605	biological_process	protein targeting
D	0007165	biological_process	signal transduction
D	0008039	biological_process	synaptic target recognition
D	0008104	biological_process	protein localization
D	0019901	molecular_function	protein kinase binding
D	0019904	molecular_function	protein domain specific binding
D	0030324	biological_process	lung development
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0031647	biological_process	regulation of protein stability
D	0031982	cellular_component	vesicle
D	0035148	biological_process	tube formation
D	0042149	biological_process	cellular response to glucose starvation
D	0042470	cellular_component	melanosome
D	0042802	molecular_function	identical protein binding
D	0043066	biological_process	negative regulation of apoptotic process
D	0043067	biological_process	regulation of programmed cell death
D	0044325	molecular_function	transmembrane transporter binding
D	0045296	molecular_function	cadherin binding
D	0045824	biological_process	negative regulation of innate immune response
D	0050815	molecular_function	phosphoserine residue binding
D	0051683	biological_process	establishment of Golgi localization
D	0070062	cellular_component	extracellular exosome
D	0070371	biological_process	ERK1 and ERK2 cascade
D	0070372	biological_process	regulation of ERK1 and ERK2 cascade
D	0072562	cellular_component	blood microparticle
D	0090128	biological_process	regulation of synapse maturation
D	0090168	biological_process	Golgi reassembly
D	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
D	0098978	cellular_component	glutamatergic synapse
D	0140297	molecular_function	DNA-binding transcription factor binding
D	0140311	molecular_function	protein sequestering activity
D	1900181	biological_process	negative regulation of protein localization to nucleus
D	1904262	biological_process	negative regulation of TORC1 signaling
E	0004059	molecular_function	aralkylamine N-acetyltransferase activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0006474	biological_process	N-terminal protein amino acid acetylation
E	0007623	biological_process	circadian rhythm
E	0009416	biological_process	response to light stimulus
E	0016746	molecular_function	acyltransferase activity
E	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
E	0030187	biological_process	melatonin biosynthetic process
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0048511	biological_process	rhythmic process
E	0071320	biological_process	cellular response to cAMP
F	0004059	molecular_function	aralkylamine N-acetyltransferase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0006474	biological_process	N-terminal protein amino acid acetylation
F	0007623	biological_process	circadian rhythm
F	0009416	biological_process	response to light stimulus
F	0016746	molecular_function	acyltransferase activity
F	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
F	0030187	biological_process	melatonin biosynthetic process
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0048511	biological_process	rhythmic process
F	0071320	biological_process	cellular response to cAMP
G	0004059	molecular_function	aralkylamine N-acetyltransferase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0006474	biological_process	N-terminal protein amino acid acetylation
G	0007623	biological_process	circadian rhythm
G	0009416	biological_process	response to light stimulus
G	0016746	molecular_function	acyltransferase activity
G	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
G	0030187	biological_process	melatonin biosynthetic process
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0048511	biological_process	rhythmic process
G	0071320	biological_process	cellular response to cAMP
H	0004059	molecular_function	aralkylamine N-acetyltransferase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0006474	biological_process	N-terminal protein amino acid acetylation
H	0007623	biological_process	circadian rhythm
H	0009416	biological_process	response to light stimulus
H	0016746	molecular_function	acyltransferase activity
H	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
H	0030187	biological_process	melatonin biosynthetic process
H	0048471	cellular_component	perinuclear region of cytoplasm
H	0048511	biological_process	rhythmic process
H	0071320	biological_process	cellular response to cAMP

Functional Information from PDB Data

site_id	AC1
Number of Residues	25
Details	BINDING SITE FOR RESIDUE COT F 400

Chain	Residue
F	PHE56
F	ARG131
F	GLN132
F	GLN133
F	GLY134
F	GLY136
F	SER137
F	MET159
F	CYS160
F	GLU161
F	ALA163
F	SER60
F	LEU164
F	PHE167
F	TYR168
F	ARG170
F	PHE188
F	HOH2129
F	CYS63
F	PRO64
F	HIS122
F	ALA123
F	LEU124
F	ALA125
F	VAL126

site_id	AC2
Number of Residues	27
Details	BINDING SITE FOR RESIDUE COT E 401

Chain	Residue
E	PHE56
E	SER60
E	ASN62
E	CYS63
E	PRO64
E	HIS122
E	ALA123
E	LEU124
E	ALA125
E	VAL126
E	ARG131
E	GLN132
E	GLN133
E	GLY134
E	GLY136
E	SER137
E	MET159
E	CYS160
E	GLU161
E	ALA163
E	LEU164
E	PHE167
E	TYR168
E	ARG170
E	HOH3192
E	HOH3491
G	ARG115

site_id	AC3
Number of Residues	27
Details	BINDING SITE FOR RESIDUE COT G 402

Chain	Residue
E	ARG115
G	PHE56
G	SER60
G	ASN62
G	CYS63
G	PRO64
G	HIS122
G	ALA123
G	LEU124
G	ALA125
G	VAL126
G	ARG131
G	GLN132
G	GLN133
G	GLY134
G	GLY136
G	SER137
G	MET159
G	CYS160
G	GLU161
G	ALA163
G	LEU164
G	PHE167
G	TYR168
G	ARG170
G	VAL183
G	HOH2839

site_id	AC4
Number of Residues	28
Details	BINDING SITE FOR RESIDUE COT H 403

Chain	Residue
H	PHE167
H	TYR168
H	ARG170
H	VAL183
H	PHE188
H	HOH1431
H	HOH1537
H	HOH1655
H	PHE56
H	SER60
H	CYS63
H	PRO64
H	HIS122
H	ALA123
H	LEU124
H	ALA125
H	VAL126
H	ARG131
H	GLN132
H	GLN133
H	GLY134
H	GLY136
H	SER137
H	MET159
H	CYS160
H	GLU161
H	ALA163
H	LEU164

Functional Information from PROSITE/UniProt

site_id	PS00796
Number of Residues	11
Details	1433_1 14-3-3 proteins signature 1. RNLLSVAYKNV

Chain	Residue	Details
A	ARG41-VAL51

site_id	PS00797
Number of Residues	20
Details	1433_2 14-3-3 proteins signature 2. YKDSTLIMQLLRDNLTLWTS

Chain	Residue	Details
A	TYR211-SER230

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:11336675, ECO:0000269\|PubMed:11884405, ECO:0000269\|PubMed:11902838

Chain	Residue	Details
E	LEU124
E	MET159
F	LEU124
F	MET159
G	LEU124
G	MET159
H	LEU124
H	MET159

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:10319816, ECO:0000269\|PubMed:11336675, ECO:0000269\|PubMed:11902838

Chain	Residue	Details
E	GLN132
E	TYR168
F	GLN132
F	TYR168
G	GLN132
G	TYR168
H	GLN132
H	TYR168

site_id	SWS_FT_FI3
Number of Residues	8
Details	SITE: Important for the catalytic mechanism; involved in substrate deprotonation => ECO:0000269\|PubMed:10319816, ECO:0000269\|PubMed:11884405

Chain	Residue	Details
E	HIS120
E	HIS122
F	HIS120
F	HIS122
G	HIS120
G	HIS122
H	HIS120
H	HIS122

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphothreonine; by PKA => ECO:0000269\|PubMed:11427721, ECO:0000269\|PubMed:14578935, ECO:0000269\|PubMed:15644438

Chain	Residue	Details
E	TPO31
F	TPO31
G	TPO31
H	TPO31

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine; by MAPK8 => ECO:0000269\|PubMed:15071501, ECO:0000269\|PubMed:15696159

Chain	Residue	Details
A	SER184
B	SER184
C	SER184
D	SER184

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:20068231

Chain	Residue	Details
A	SER207
B	SER207
C	SER207
D	SER207

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P63102

Chain	Residue	Details
A	SER210
B	SER210
C	SER210
D	SER210

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphothreonine; by CK1 => ECO:0000269\|PubMed:31024343, ECO:0000269\|PubMed:9360956, ECO:0007744\|PubMed:20068231

Chain	Residue	Details
A	THR232
B	THR232
C	THR232
D	THR232

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1b6b

Chain	Residue	Details
E	SER97
E	LEU111
E	HIS122
E	LEU124
E	TYR168

site_id	CSA2
Number of Residues	5
Details	Annotated By Reference To The Literature 1b6b

Chain	Residue	Details
F	SER97
F	LEU111
F	HIS122
F	LEU124
F	TYR168

site_id	CSA3
Number of Residues	5
Details	Annotated By Reference To The Literature 1b6b

Chain	Residue	Details
G	SER97
G	LEU111
G	HIS122
G	LEU124
G	TYR168

site_id	CSA4
Number of Residues	5
Details	Annotated By Reference To The Literature 1b6b

Chain	Residue	Details
H	SER97
H	LEU111
H	HIS122
H	LEU124
H	TYR168

site_id	MCSA1
Number of Residues	5
Details	M-CSA 22

Chain	Residue	Details
E	SER97	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
E	LEU111	hydrogen bond acceptor, steric role
E	HIS122	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
E	LEU124	electrostatic stabiliser, hydrogen bond donor
E	TYR168	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA2
Number of Residues	5
Details	M-CSA 22

Chain	Residue	Details
F	SER97	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
F	LEU111	hydrogen bond acceptor, steric role
F	HIS122	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
F	LEU124	electrostatic stabiliser, hydrogen bond donor
F	TYR168	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA3
Number of Residues	5
Details	M-CSA 22

Chain	Residue	Details
G	SER97	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
G	LEU111	hydrogen bond acceptor, steric role
G	HIS122	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
G	LEU124	electrostatic stabiliser, hydrogen bond donor
G	TYR168	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA4
Number of Residues	5
Details	M-CSA 22

Chain	Residue	Details
H	SER97	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
H	LEU111	hydrogen bond acceptor, steric role
H	HIS122	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
H	LEU124	electrostatic stabiliser, hydrogen bond donor
H	TYR168	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)