1IB1
CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERASE COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-10 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 74.717, 75.078, 101.780 |
Unit cell angles | 90.14, 90.06, 63.04 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1A4O AND 1CJW |
RMSD bond length | 0.008 |
RMSD bond angle | 1.550 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.780 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.102 | 0.251 |
Total number of observations | 78001 * | |
Number of reflections | 44944 | |
<I/σ(I)> | 5.6 | 2.09 |
Completeness [%] | 84.9 | 72 |
Redundancy | 1.74 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | PEG 4000, magnesium chloride, lithium sulfate, DTT, EDTA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 20 (mM) | |
3 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
4 | 1 | reservoir | 20 (mM) |