Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1I0X

RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001411	cellular_component	hyphal tip
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0030428	cellular_component	cell septum
A	0046589	molecular_function	ribonuclease T1 activity
B	0001411	cellular_component	hyphal tip
B	0003723	molecular_function	RNA binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004521	molecular_function	RNA endonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0016787	molecular_function	hydrolase activity
B	0016829	molecular_function	lyase activity
B	0030428	cellular_component	cell septum
B	0046589	molecular_function	ribonuclease T1 activity
C	0001411	cellular_component	hyphal tip
C	0003723	molecular_function	RNA binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0004521	molecular_function	RNA endonuclease activity
C	0004540	molecular_function	RNA nuclease activity
C	0016787	molecular_function	hydrolase activity
C	0016829	molecular_function	lyase activity
C	0030428	cellular_component	cell septum
C	0046589	molecular_function	ribonuclease T1 activity
D	0001411	cellular_component	hyphal tip
D	0003723	molecular_function	RNA binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004521	molecular_function	RNA endonuclease activity
D	0004540	molecular_function	RNA nuclease activity
D	0016787	molecular_function	hydrolase activity
D	0016829	molecular_function	lyase activity
D	0030428	cellular_component	cell septum
D	0046589	molecular_function	ribonuclease T1 activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA C 701

Chain	Residue
C	GLY47
C	ASP49
C	HOH719
C	HOH720
C	HOH721
D	TYR45
D	HOH796

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA D 702

Chain	Residue
D	GLY47
D	ASP49
D	HOH705
D	HOH721
D	HOH832
C	TYR45
C	HOH763

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA B 703

Chain	Residue
B	PHE50
B	HOH724
B	HOH729
D	CYS2
D	HOH741
D	HOH852
D	HOH853

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA D 704

Chain	Residue
D	HIS92
D	ALA95
D	HOH733
D	HOH743
D	HOH828
D	HOH866
D	HOH867

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 705

Chain	Residue
B	HOH721
B	HOH746
B	HOH748
B	HOH782
B	HOH839
B	HOH842

site_id	AC6
Number of Residues	14
Details	BINDING SITE FOR RESIDUE 2GP A 601

Chain	Residue
A	ASN36
A	TYR38
A	HIS40
A	LYS41
A	TYR42
A	ASN43
A	ASN44
A	TYR45
A	GLU46
A	GLU58
A	ASN98
A	PHE100
A	HOH612
A	HOH675

site_id	AC7
Number of Residues	16
Details	BINDING SITE FOR RESIDUE 2GP B 602

Chain	Residue
B	ASN36
B	TYR38
B	HIS40
B	LYS41
B	TYR42
B	ASN43
B	ASN44
B	TYR45
B	GLU46
B	GLU58
B	ASN98
B	PHE100
B	HOH719
B	HOH754
B	HOH767
C	HOH848

site_id	AC8
Number of Residues	16
Details	BINDING SITE FOR RESIDUE 2GP C 603

Chain	Residue
A	SER53
A	HOH606
A	HOH611
B	HOH721
B	HOH839
C	HIS40
C	LYS41
C	TYR42
C	ASN43
C	ASN44
C	TYR45
C	GLU46
C	ASN99
C	PHE100
C	HOH733
C	HOH745

site_id	AC9
Number of Residues	23
Details	BINDING SITE FOR RESIDUE 2GP C 604

Chain	Residue
C	HOH711
C	HOH741
C	HOH765
C	HOH850
A	SER53
A	SER54
A	PRO55
A	ASN81
A	GLU82
A	ASN83
A	HOH606
A	HOH611
C	ASN36
C	TYR38
C	HIS40
C	GLU58
C	SER72
C	PRO73
C	GLY74
C	ARG77
C	HIS92
C	ASN98
C	PHE100

site_id	BC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE 2GP D 605

Chain	Residue
D	TYR38
D	HIS40
D	LYS41
D	TYR42
D	ASN43
D	ASN44
D	TYR45
D	GLU46
D	GLU58
D	ASN98
D	PHE100
D	HOH738
D	HOH815
D	HOH823

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"evidences":[{"source":"PubMed","id":"2844811","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"2844811","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"description":"Proton donor"}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
A	GLU58
A	HIS40
A	HIS92

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
B	GLU58
B	HIS40
B	HIS92

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
C	GLU58
C	HIS40
C	HIS92

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
D	GLU58
D	HIS40
D	HIS92

site_id	MCSA1
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
A	TYR38	electrostatic stabiliser
A	HIS40	proton shuttle (general acid/base)
A	GLU58	proton shuttle (general acid/base)
A	ARG77	electrostatic stabiliser
A	HIS92	proton shuttle (general acid/base)
A	PHE100	electrostatic stabiliser

site_id	MCSA2
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
B	TYR38	electrostatic stabiliser
B	HIS40	proton shuttle (general acid/base)
B	GLU58	proton shuttle (general acid/base)
B	ARG77	electrostatic stabiliser
B	HIS92	proton shuttle (general acid/base)
B	PHE100	electrostatic stabiliser

site_id	MCSA3
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
C	TYR38	electrostatic stabiliser
C	HIS40	proton shuttle (general acid/base)
C	GLU58	proton shuttle (general acid/base)
C	ARG77	electrostatic stabiliser
C	HIS92	proton shuttle (general acid/base)
C	PHE100	electrostatic stabiliser

site_id	MCSA4
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
D	TYR38	electrostatic stabiliser
D	HIS40	proton shuttle (general acid/base)
D	GLU58	proton shuttle (general acid/base)
D	ARG77	electrostatic stabiliser
D	HIS92	proton shuttle (general acid/base)
D	PHE100	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)