1I0X

RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-11
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	58.271, 59.696, 100.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.650
R-factor	0.1903 *
Rwork	0.190
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	1.571 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.700
High resolution limit [Å]	1.650	1.650
Rmerge	0.100	0.534
Total number of observations	228296 *
Number of reflections	41132 *	3920 *
<I/σ(I)>	12.81	2.72
Completeness [%]	95.7	92.4
Redundancy	5.55	5.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	MPD, acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K