1I0X
RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.271, 59.696, 100.136 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.650 |
R-factor | 0.1903 * |
Rwork | 0.190 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.571 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.700 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.100 | 0.534 |
Total number of observations | 228296 * | |
Number of reflections | 41132 * | 3920 * |
<I/σ(I)> | 12.81 | 2.72 |
Completeness [%] | 95.7 | 92.4 |
Redundancy | 5.55 | 5.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | MPD, acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |