Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1HR8

Yeast Mitochondrial Processing Peptidase beta-E73Q Mutant Complexed with Cytochrome C Oxidase IV Signal Peptide

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004222	molecular_function	metalloendopeptidase activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0006508	biological_process	proteolysis
A	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
A	0017087	cellular_component	mitochondrial processing peptidase complex
A	0046872	molecular_function	metal ion binding
A	0061133	molecular_function	endopeptidase activator activity
B	0004222	molecular_function	metalloendopeptidase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0006508	biological_process	proteolysis
B	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
B	0008233	molecular_function	peptidase activity
B	0008237	molecular_function	metallopeptidase activity
B	0008270	molecular_function	zinc ion binding
B	0016787	molecular_function	hydrolase activity
B	0017087	cellular_component	mitochondrial processing peptidase complex
B	0046872	molecular_function	metal ion binding
C	0004222	molecular_function	metalloendopeptidase activity
C	0005515	molecular_function	protein binding
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0006508	biological_process	proteolysis
C	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
C	0017087	cellular_component	mitochondrial processing peptidase complex
C	0046872	molecular_function	metal ion binding
C	0061133	molecular_function	endopeptidase activator activity
D	0004222	molecular_function	metalloendopeptidase activity
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0006508	biological_process	proteolysis
D	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
D	0008233	molecular_function	peptidase activity
D	0008237	molecular_function	metallopeptidase activity
D	0008270	molecular_function	zinc ion binding
D	0016787	molecular_function	hydrolase activity
D	0017087	cellular_component	mitochondrial processing peptidase complex
D	0046872	molecular_function	metal ion binding
E	0004222	molecular_function	metalloendopeptidase activity
E	0005515	molecular_function	protein binding
E	0005739	cellular_component	mitochondrion
E	0005759	cellular_component	mitochondrial matrix
E	0006508	biological_process	proteolysis
E	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
E	0017087	cellular_component	mitochondrial processing peptidase complex
E	0046872	molecular_function	metal ion binding
E	0061133	molecular_function	endopeptidase activator activity
F	0004222	molecular_function	metalloendopeptidase activity
F	0005515	molecular_function	protein binding
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0006508	biological_process	proteolysis
F	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
F	0008233	molecular_function	peptidase activity
F	0008237	molecular_function	metallopeptidase activity
F	0008270	molecular_function	zinc ion binding
F	0016787	molecular_function	hydrolase activity
F	0017087	cellular_component	mitochondrial processing peptidase complex
F	0046872	molecular_function	metal ion binding
G	0004222	molecular_function	metalloendopeptidase activity
G	0005515	molecular_function	protein binding
G	0005739	cellular_component	mitochondrion
G	0005759	cellular_component	mitochondrial matrix
G	0006508	biological_process	proteolysis
G	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
G	0017087	cellular_component	mitochondrial processing peptidase complex
G	0046872	molecular_function	metal ion binding
G	0061133	molecular_function	endopeptidase activator activity
H	0004222	molecular_function	metalloendopeptidase activity
H	0005515	molecular_function	protein binding
H	0005739	cellular_component	mitochondrion
H	0005759	cellular_component	mitochondrial matrix
H	0006508	biological_process	proteolysis
H	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
H	0008233	molecular_function	peptidase activity
H	0008237	molecular_function	metallopeptidase activity
H	0008270	molecular_function	zinc ion binding
H	0016787	molecular_function	hydrolase activity
H	0017087	cellular_component	mitochondrial processing peptidase complex
H	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 501

Chain	Residue
B	HIS70
B	HIS74
B	GLU150
O	HOH1

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 502

Chain	Residue
D	HIS70
D	HIS74
D	GLU150
P	HOH26

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN F 503

Chain	Residue
F	HIS70
F	HIS74
F	GLU150
F	HOH3

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN H 504

Chain	Residue
H	HOH4
H	HIS70
H	HIS74
H	GLU150

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EPE A 489

Chain	Residue
A	TRP147
A	PRO175
A	ARG176
E	PRO175
E	ARG176

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EPE G 489

Chain	Residue
C	TRP147
C	PRO175
C	ARG176
G	PRO175
G	ARG176

Functional Information from PROSITE/UniProt

site_id	PS00143
Number of Residues	24
Details	INSULINASE Insulinase family, zinc-binding region signature. GsrfegrnlkGcTHILDRLaFkST

Chain	Residue	Details
A	GLY49-THR72

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000269\|PubMed:11470436, ECO:0007744\|PDB:1HR6

Chain	Residue	Details
B	GLN73
D	GLN73
F	GLN73
H	GLN73

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU10096, ECO:0000269\|PubMed:11470436, ECO:0000269\|PubMed:9654444, ECO:0007744\|PDB:1HR6, ECO:0007744\|PDB:1HR7, ECO:0007744\|PDB:1HR8, ECO:0007744\|PDB:1HR9

Chain	Residue	Details
B	HIS70
D	HIS70
F	HIS70
H	HIS70

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU10096, ECO:0000269\|PubMed:11470436, ECO:0007744\|PDB:1HR6, ECO:0007744\|PDB:1HR7, ECO:0007744\|PDB:1HR8, ECO:0007744\|PDB:1HR9

Chain	Residue	Details
B	HIS74
B	GLU150
D	HIS74
D	GLU150
F	HIS74
F	GLU150
H	HIS74
H	GLU150

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
B	SER243
D	SER243
F	SER243
H	SER243

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 657

Chain	Residue	Details
B	HIS70	metal ligand
B	GLN73	proton acceptor, proton donor
B	HIS74	hydrogen bond donor, metal ligand
B	GLU143	electrostatic stabiliser, hydrogen bond acceptor
B	GLU150	metal ligand

site_id	MCSA2
Number of Residues	5
Details	M-CSA 657

Chain	Residue	Details
D	HIS70	metal ligand
D	GLN73	proton acceptor, proton donor
D	HIS74	hydrogen bond donor, metal ligand
D	GLU143	electrostatic stabiliser, hydrogen bond acceptor
D	GLU150	metal ligand

site_id	MCSA3
Number of Residues	5
Details	M-CSA 657

Chain	Residue	Details
F	HIS70	metal ligand
F	GLN73	proton acceptor, proton donor
F	HIS74	hydrogen bond donor, metal ligand
F	GLU143	electrostatic stabiliser, hydrogen bond acceptor
F	GLU150	metal ligand

site_id	MCSA4
Number of Residues	5
Details	M-CSA 657

Chain	Residue	Details
H	HIS70	metal ligand
H	GLN73	proton acceptor, proton donor
H	HIS74	hydrogen bond donor, metal ligand
H	GLU143	electrostatic stabiliser, hydrogen bond acceptor
H	GLU150	metal ligand

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)