1HR8

Yeast Mitochondrial Processing Peptidase beta-E73Q Mutant Complexed with Cytochrome C Oxidase IV Signal Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	105
Detector technology	CCD
Collection date	2000-07-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9430
Spacegroup name	P 21 21 21
Unit cell lengths	134.260, 178.120, 202.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.630 - 2.700
R-factor	0.229
Rwork	0.229
R-free	0.26400
Starting model (for MR)	PDB ID 1HR6
RMSD bond length	0.013
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.079	0.543
Total number of observations	820094 *
Number of reflections	134234
<I/σ(I)>	23.4	3.5
Completeness [%]	99.9	100
Redundancy	6.1	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-14 (mg/ml)
10	1	reservoir	n-dodecylmaltoside	0.2 (mM)
11	1	reservoir	sodium azide	2 (mM)
2	1	drop	HEPES	50 (mM)
3	1	drop	glycerol	30 (%(v/v))
4	1	drop	n-dodecylmaltoside	0.2 (mM)
5	1	reservoir	PEG10000	5-9 (%(w/v))
6	1	reservoir	ethylene glycol	35 (%(v/v))
7	1	reservoir	MPD	6 (%(v/v))
8	1	reservoir	sodium citrate	25 (mM)
9	1	reservoir	benzamidine	2 (%(w/v))