1HFW

X-ray structure of the complex between Erwinia chrysanthemi L-asparaginase and L-Glutamate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004067	molecular_function	asparaginase activity
A	0006520	biological_process	amino acid metabolic process
A	0006528	biological_process	asparagine metabolic process
A	0016787	molecular_function	hydrolase activity
B	0004067	molecular_function	asparaginase activity
B	0006520	biological_process	amino acid metabolic process
B	0006528	biological_process	asparagine metabolic process
B	0016787	molecular_function	hydrolase activity
C	0004067	molecular_function	asparaginase activity
C	0006520	biological_process	amino acid metabolic process
C	0006528	biological_process	asparagine metabolic process
C	0016787	molecular_function	hydrolase activity
D	0004067	molecular_function	asparaginase activity
D	0006520	biological_process	amino acid metabolic process
D	0006528	biological_process	asparagine metabolic process
D	0016787	molecular_function	hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GLU A 350

Chain	Residue
A	GLY14
A	HOH2215
A	HOH2216
C	SER254
A	THR15
A	ALA61
A	SER62
A	GLU63
A	GLY94
A	THR95
A	ASP96
A	ALA120

---

site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GLU B 350

Chain	Residue
B	GLY14
B	THR15
B	ALA61
B	SER62
B	GLU63
B	GLY94
B	THR95
B	ASP96
B	HOH2249
B	HOH2250
B	HOH2251
D	SER254

---

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE GLU C 350

Chain	Residue
A	SER254
C	GLY14
C	THR15
C	ALA61
C	SER62
C	GLU63
C	GLY94
C	THR95
C	ASP96
C	ALA120
C	HOH2022
C	HOH2233
C	HOH2235

---

site_id	AC4
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GLU D 350

Chain	Residue
B	SER254
D	GLY14
D	THR15
D	ALA61
D	SER62
D	GLU63
D	GLY94
D	THR95
D	ASP96
D	HOH2257
D	HOH2258
D	HOH2259

---

Functional Information from PROSITE/UniProt

site_id	PS00144
Number of Residues	9
Details	ASN_GLN_ASE_1 Asparaginase / glutaminase active site signature 1. IlATGGTIA

Chain	Residue	Details
A	ILE9-ALA17

---

site_id	PS00917
Number of Residues	11
Details	ASN_GLN_ASE_2 Asparaginase / glutaminase active site signature 2. GvVitHGTDTV

Chain	Residue	Details
A	GLY88-VAL98

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"O-isoaspartyl threonine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU10099","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10100","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11755201","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8348975","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
A	THR15
A	THR95
A	ASP96
A	LYS168

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
B	THR15
B	THR95
B	ASP96
B	LYS168

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
C	THR15
C	THR95
C	ASP96
C	LYS168

---

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
D	THR15
D	THR95
D	ASP96
D	LYS168

---

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
A	VAL284
C	THR15
C	THR95

---

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
D	THR15
D	THR95
B	VAL284

---

site_id	CSA7
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
A	THR15
A	THR95
C	VAL284

---

site_id	CSA8
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
D	VAL284
B	THR15
B	THR95

---