1HFW

X-ray structure of the complex between Erwinia chrysanthemi L-asparaginase and L-Glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1998-08-15
Detector	MAR scanner 345 mm plate
Spacegroup name	C 1 2 1
Unit cell lengths	108.470, 91.700, 130.620
Unit cell angles	90.00, 91.94, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.168
Rwork	0.168
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PREVIUOSLY PUBLISHED STRUCTURE OF ERWINIA CHRYSANTHEMI L-ASPARAGINSE (MILLER ET AL. FEBS LETT. 1993
RMSD bond length	0.006
RMSD bond angle	23.590 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.076 *	0.337 *
Total number of observations	270156 *
Number of reflections	102023
<I/σ(I)>	7.4	2.8
Completeness [%]	86.0	65.4
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.4		Miller, M., (1993) FEBS Lett., 328, 275. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	50 (%)
2	1	reservoir	CHES	0.1 (M)
3	1	reservoir	PEG400	2 (%(w/v))