1H4J

Methylobacterium extorquens methanol dehydrogenase D303E mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005509	molecular_function	calcium ion binding
A	0005886	cellular_component	plasma membrane
A	0016020	cellular_component	membrane
A	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
A	0030288	cellular_component	outer membrane-bounded periplasmic space
A	0052933	molecular_function	alcohol dehydrogenase (cytochrome c(L)) activity
A	0070968	molecular_function	pyrroloquinoline quinone binding
B	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
B	0015946	biological_process	methanol oxidation
B	0042597	cellular_component	periplasmic space
B	0052933	molecular_function	alcohol dehydrogenase (cytochrome c(L)) activity
C	0003824	molecular_function	catalytic activity
C	0005509	molecular_function	calcium ion binding
C	0005886	cellular_component	plasma membrane
C	0016020	cellular_component	membrane
C	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
C	0030288	cellular_component	outer membrane-bounded periplasmic space
C	0052933	molecular_function	alcohol dehydrogenase (cytochrome c(L)) activity
C	0070968	molecular_function	pyrroloquinoline quinone binding
D	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
D	0015946	biological_process	methanol oxidation
D	0042597	cellular_component	periplasmic space
D	0052933	molecular_function	alcohol dehydrogenase (cytochrome c(L)) activity
E	0003824	molecular_function	catalytic activity
E	0005509	molecular_function	calcium ion binding
E	0005886	cellular_component	plasma membrane
E	0016020	cellular_component	membrane
E	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
E	0030288	cellular_component	outer membrane-bounded periplasmic space
E	0052933	molecular_function	alcohol dehydrogenase (cytochrome c(L)) activity
E	0070968	molecular_function	pyrroloquinoline quinone binding
F	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
F	0015946	biological_process	methanol oxidation
F	0042597	cellular_component	periplasmic space
F	0052933	molecular_function	alcohol dehydrogenase (cytochrome c(L)) activity
G	0003824	molecular_function	catalytic activity
G	0005509	molecular_function	calcium ion binding
G	0005886	cellular_component	plasma membrane
G	0016020	cellular_component	membrane
G	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
G	0030288	cellular_component	outer membrane-bounded periplasmic space
G	0052933	molecular_function	alcohol dehydrogenase (cytochrome c(L)) activity
G	0070968	molecular_function	pyrroloquinoline quinone binding
H	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
H	0015946	biological_process	methanol oxidation
H	0042597	cellular_component	periplasmic space
H	0052933	molecular_function	alcohol dehydrogenase (cytochrome c(L)) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA A 701

Chain	Residue
A	GLU177
A	ASN261
A	GLU303
A	PQQ601

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA C 701

Chain	Residue
C	GLU177
C	ASN261
C	GLU303
C	PQQ601

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA E 701

Chain	Residue
E	ASN261
E	GLU303
E	PQQ601
E	GLU177

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA G 701

Chain	Residue
G	GLU177
G	ASN261
G	GLU303
G	PQQ601

---

site_id	AC5
Number of Residues	19
Details	BINDING SITE FOR RESIDUE PQQ A 601

Chain	Residue
A	GLU55
A	CYS104
A	VAL107
A	ARG109
A	THR159
A	SER174
A	GLY175
A	ALA176
A	GLU177
A	THR241
A	TRP243
A	ASN261
A	GLU303
A	ARG331
A	ASN394
A	TRP476
A	GLY539
A	TRP540
A	CA701

---

site_id	AC6
Number of Residues	19
Details	BINDING SITE FOR RESIDUE PQQ C 601

Chain	Residue
C	GLU55
C	CYS104
C	VAL107
C	ARG109
C	THR159
C	SER174
C	GLY175
C	ALA176
C	GLU177
C	THR241
C	TRP243
C	ASN261
C	GLU303
C	ARG331
C	ASN394
C	TRP476
C	GLY539
C	TRP540
C	CA701

---

site_id	AC7
Number of Residues	19
Details	BINDING SITE FOR RESIDUE PQQ E 601

Chain	Residue
E	GLU55
E	CYS104
E	VAL107
E	ARG109
E	THR159
E	SER174
E	GLY175
E	ALA176
E	GLU177
E	THR241
E	TRP243
E	ASN261
E	GLU303
E	ARG331
E	ASN394
E	TRP476
E	GLY539
E	TRP540
E	CA701

---

site_id	AC8
Number of Residues	19
Details	BINDING SITE FOR RESIDUE PQQ G 601

Chain	Residue
G	GLU55
G	CYS104
G	VAL107
G	ARG109
G	THR159
G	SER174
G	GLY175
G	ALA176
G	GLU177
G	THR241
G	TRP243
G	ASN261
G	GLU303
G	ARG331
G	ASN394
G	TRP476
G	GLY539
G	TRP540
G	CA701

---

Functional Information from PROSITE/UniProt

site_id	PS00363
Number of Residues	29
Details	BACTERIAL_PQQ_1 Bacterial quinoprotein dehydrogenases signature 1. NWvmpGKnydsnnFSdlkqINkgNVkqLR

Chain	Residue	Details
A	ASN13-ARG41

---

site_id	PS00364
Number of Residues	22
Details	BACTERIAL_PQQ_2 Bacterial quinoprotein dehydrogenases signature 2. WgwyaYDpgtNLIYFgtGnpAP

Chain	Residue	Details
A	TRP243-PRO264

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	120
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	44
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1g72

Chain	Residue	Details
A	GLU303

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1g72

Chain	Residue	Details
C	GLU303

---

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1g72

Chain	Residue	Details
E	GLU303

---

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1g72

Chain	Residue	Details
G	GLU303

---