1H4J
Methylobacterium extorquens methanol dehydrogenase D303E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.850, 61.020, 212.680 |
| Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD-TYPE STRUCTURE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MOLREP |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.094 | 0.265 |
| Total number of observations | 134792 * | |
| Number of reflections | 48502 | |
| <I/σ(I)> | 6.9 | 2.6 |
| Completeness [%] | 91.5 | 81.4 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9.2 * | 4 * | pH 9.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 14.5 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | drop | Tris | 5 (mM) |
