Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1H2U

Structure of the human nuclear cap-binding-complex (CBC) in complex with a cap analogue m7GpppG

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
A	0000245	biological_process	spliceosomal complex assembly
A	0000339	molecular_function	RNA cap binding
A	0000340	molecular_function	RNA 7-methylguanosine cap binding
A	0000380	biological_process	alternative mRNA splicing, via spliceosome
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0002191	biological_process	cap-dependent translational initiation
A	0003723	molecular_function	RNA binding
A	0003729	molecular_function	mRNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0005846	cellular_component	nuclear cap binding complex
A	0006370	biological_process	7-methylguanosine mRNA capping
A	0006397	biological_process	mRNA processing
A	0006401	biological_process	RNA catabolic process
A	0006406	biological_process	mRNA export from nucleus
A	0006408	biological_process	snRNA export from nucleus
A	0006417	biological_process	regulation of translation
A	0006446	biological_process	regulation of translational initiation
A	0008334	biological_process	histone mRNA metabolic process
A	0008380	biological_process	RNA splicing
A	0016070	biological_process	RNA metabolic process
A	0016071	biological_process	mRNA metabolic process
A	0030307	biological_process	positive regulation of cell growth
A	0031047	biological_process	regulatory ncRNA-mediated gene silencing
A	0031053	biological_process	primary miRNA processing
A	0031124	biological_process	mRNA 3'-end processing
A	0031442	biological_process	positive regulation of mRNA 3'-end processing
A	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
A	0034518	cellular_component	RNA cap binding complex
A	0035194	biological_process	regulatory ncRNA-mediated post-transcriptional gene silencing
A	0035195	biological_process	miRNA-mediated post-transcriptional gene silencing
A	0042789	biological_process	mRNA transcription by RNA polymerase II
A	0048026	biological_process	positive regulation of mRNA splicing, via spliceosome
A	0050684	biological_process	regulation of mRNA processing
A	0051028	biological_process	mRNA transport
A	0051607	biological_process	defense response to virus
A	0060090	molecular_function	molecular adaptor activity
A	1905216	biological_process	positive regulation of RNA binding
A	1990904	cellular_component	ribonucleoprotein complex
B	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
B	0000245	biological_process	spliceosomal complex assembly
B	0000339	molecular_function	RNA cap binding
B	0000340	molecular_function	RNA 7-methylguanosine cap binding
B	0000380	biological_process	alternative mRNA splicing, via spliceosome
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0002191	biological_process	cap-dependent translational initiation
B	0003723	molecular_function	RNA binding
B	0003729	molecular_function	mRNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0005846	cellular_component	nuclear cap binding complex
B	0006370	biological_process	7-methylguanosine mRNA capping
B	0006397	biological_process	mRNA processing
B	0006401	biological_process	RNA catabolic process
B	0006406	biological_process	mRNA export from nucleus
B	0006408	biological_process	snRNA export from nucleus
B	0006417	biological_process	regulation of translation
B	0006446	biological_process	regulation of translational initiation
B	0008334	biological_process	histone mRNA metabolic process
B	0008380	biological_process	RNA splicing
B	0016070	biological_process	RNA metabolic process
B	0016071	biological_process	mRNA metabolic process
B	0030307	biological_process	positive regulation of cell growth
B	0031047	biological_process	regulatory ncRNA-mediated gene silencing
B	0031053	biological_process	primary miRNA processing
B	0031124	biological_process	mRNA 3'-end processing
B	0031442	biological_process	positive regulation of mRNA 3'-end processing
B	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
B	0034518	cellular_component	RNA cap binding complex
B	0035194	biological_process	regulatory ncRNA-mediated post-transcriptional gene silencing
B	0035195	biological_process	miRNA-mediated post-transcriptional gene silencing
B	0042789	biological_process	mRNA transcription by RNA polymerase II
B	0048026	biological_process	positive regulation of mRNA splicing, via spliceosome
B	0050684	biological_process	regulation of mRNA processing
B	0051028	biological_process	mRNA transport
B	0051607	biological_process	defense response to virus
B	0060090	molecular_function	molecular adaptor activity
B	1905216	biological_process	positive regulation of RNA binding
B	1990904	cellular_component	ribonucleoprotein complex
X	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
X	0000339	molecular_function	RNA cap binding
X	0000340	molecular_function	RNA 7-methylguanosine cap binding
X	0000380	biological_process	alternative mRNA splicing, via spliceosome
X	0000398	biological_process	mRNA splicing, via spliceosome
X	0002191	biological_process	cap-dependent translational initiation
X	0003676	molecular_function	nucleic acid binding
X	0003677	molecular_function	DNA binding
X	0003723	molecular_function	RNA binding
X	0003729	molecular_function	mRNA binding
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005846	cellular_component	nuclear cap binding complex
X	0006397	biological_process	mRNA processing
X	0006406	biological_process	mRNA export from nucleus
X	0006408	biological_process	snRNA export from nucleus
X	0006417	biological_process	regulation of translation
X	0006446	biological_process	regulation of translational initiation
X	0008334	biological_process	histone mRNA metabolic process
X	0008380	biological_process	RNA splicing
X	0016071	biological_process	mRNA metabolic process
X	0017069	molecular_function	snRNA binding
X	0031047	biological_process	regulatory ncRNA-mediated gene silencing
X	0031053	biological_process	primary miRNA processing
X	0031124	biological_process	mRNA 3'-end processing
X	0031442	biological_process	positive regulation of mRNA 3'-end processing
X	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
X	0034518	cellular_component	RNA cap binding complex
X	0035194	biological_process	regulatory ncRNA-mediated post-transcriptional gene silencing
X	0035195	biological_process	miRNA-mediated post-transcriptional gene silencing
X	0042789	biological_process	mRNA transcription by RNA polymerase II
X	0045292	biological_process	mRNA cis splicing, via spliceosome
X	0046833	biological_process	positive regulation of RNA export from nucleus
X	0051028	biological_process	mRNA transport
Y	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Y	0000339	molecular_function	RNA cap binding
Y	0000340	molecular_function	RNA 7-methylguanosine cap binding
Y	0000380	biological_process	alternative mRNA splicing, via spliceosome
Y	0000398	biological_process	mRNA splicing, via spliceosome
Y	0002191	biological_process	cap-dependent translational initiation
Y	0003676	molecular_function	nucleic acid binding
Y	0003677	molecular_function	DNA binding
Y	0003723	molecular_function	RNA binding
Y	0003729	molecular_function	mRNA binding
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005654	cellular_component	nucleoplasm
Y	0005737	cellular_component	cytoplasm
Y	0005829	cellular_component	cytosol
Y	0005846	cellular_component	nuclear cap binding complex
Y	0006397	biological_process	mRNA processing
Y	0006406	biological_process	mRNA export from nucleus
Y	0006408	biological_process	snRNA export from nucleus
Y	0006417	biological_process	regulation of translation
Y	0006446	biological_process	regulation of translational initiation
Y	0008334	biological_process	histone mRNA metabolic process
Y	0008380	biological_process	RNA splicing
Y	0016071	biological_process	mRNA metabolic process
Y	0017069	molecular_function	snRNA binding
Y	0031047	biological_process	regulatory ncRNA-mediated gene silencing
Y	0031053	biological_process	primary miRNA processing
Y	0031124	biological_process	mRNA 3'-end processing
Y	0031442	biological_process	positive regulation of mRNA 3'-end processing
Y	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
Y	0034518	cellular_component	RNA cap binding complex
Y	0035194	biological_process	regulatory ncRNA-mediated post-transcriptional gene silencing
Y	0035195	biological_process	miRNA-mediated post-transcriptional gene silencing
Y	0042789	biological_process	mRNA transcription by RNA polymerase II
Y	0045292	biological_process	mRNA cis splicing, via spliceosome
Y	0046833	biological_process	positive regulation of RNA export from nucleus
Y	0051028	biological_process	mRNA transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE GDP X1153

Chain	Residue
X	TYR20
Y	GLN19
Y	ARG21
Y	GLN23
Y	ASN29
X	ARG127
X	VAL134
X	ARG135
X	7MG1154
X	HOH2127
X	HOH2128
X	HOH2129
X	HOH2130

site_id	AC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE 7MG X1154

Chain	Residue
X	TYR20
X	ASP22
X	TYR43
X	PHE83
X	ARG112
X	ASP114
X	TRP115
X	ASP116
X	ARG123
X	TYR125
X	GLY126
X	ARG127
X	GLN133
X	VAL134
X	ARG135
X	GDP1153
X	HOH2130

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE GDP Y1152

Chain	Residue
X	GLN23
X	PHE25
X	GLY27
X	HOH2022
Y	TYR20
Y	ARG127
Y	VAL134
Y	ARG135
Y	TYR138
Y	7MG1153
Y	HOH2138
Y	HOH2139
Y	HOH2140

site_id	AC4
Number of Residues	16
Details	BINDING SITE FOR RESIDUE 7MG Y1153

Chain	Residue
Y	TYR20
Y	TYR43
Y	PHE83
Y	ARG112
Y	ASP114
Y	TRP115
Y	ASP116
Y	ARG123
Y	TYR125
Y	GLY126
Y	ARG127
Y	GLN133
Y	VAL134
Y	ARG135
Y	GDP1152
Y	HOH2140

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:12374755, ECO:0000269\|PubMed:12434151

Chain	Residue	Details
X	TYR20
X	TYR43
Y	TYR20
Y	TYR43

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING:

Chain	Residue	Details
X	ARG112
X	ARG123
X	GLN133
Y	ARG112
Y	ARG123
Y	GLN133

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N-acetylserine => ECO:0007744\|PubMed:22814378

Chain	Residue	Details
X	SER2
Y	SER2

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
X	SER13
Y	SER13
B	LYS204
B	ARG746

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17525332

Chain	Residue	Details
X	SER18
Y	SER18
B	GLY732

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Omega-N-methylarginine => ECO:0007744\|PubMed:24129315

Chain	Residue	Details
X	ARG146
Y	ARG146

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)