1GXS

Crystal Structure of Hydroxynitrile Lyase from Sorghum bicolor in Complex with Inhibitor Benzoic Acid: a novel cyanogenic enzyme

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004185	molecular_function	serine-type carboxypeptidase activity
A	0006508	biological_process	proteolysis
B	0004185	molecular_function	serine-type carboxypeptidase activity
B	0006508	biological_process	proteolysis
C	0004185	molecular_function	serine-type carboxypeptidase activity
C	0006508	biological_process	proteolysis
D	0004185	molecular_function	serine-type carboxypeptidase activity
D	0006508	biological_process	proteolysis

Functional Information from PROSITE/UniProt

site_id	PS00560
Number of Residues	18
Details	CARBOXYPEPT_SER_HIS Serine carboxypeptidases, histidine active site. LtyVtVrGAGHlVPvhrP

Chain	Residue	Details
B	LEU404-PRO421

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: ACT_SITE => ECO:0000250|UniProtKB:P08819

Chain	Residue	Details
B	ASP359
B	HIS414
D	ASP359
D	HIS414

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site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P08819

Chain	Residue	Details
B	ARG410
D	ARG410
C	ASN61
C	GLU157

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site_id	SWS_FT_FI3
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255|PROSITE-ProRule:PRU00498, ECO:0000269|PubMed:12356304, ECO:0007744|PDB:1GXS

Chain	Residue	Details
B	ASN310
D	ASN310

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 12356304

Chain	Residue	Details
A	SER158
A	TRP270

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site_id	CSA2
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 12356304

Chain	Residue	Details
C	SER158
C	TRP270

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site_id	MCSA1
Number of Residues
Details	M-CSA 382

Chain	Residue	Details
A	SER158	electrostatic stabiliser, modifies pKa
A	TRP270	proton shuttle (general acid/base)

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site_id	MCSA2
Number of Residues
Details	M-CSA 382

Chain	Residue	Details
C	SER158	electrostatic stabiliser, modifies pKa
C	TRP270	proton shuttle (general acid/base)

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